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Literature summary for 1.2.3.4 extracted from
- Isolation, purification, immobilization of oxalate oxidase and its clinical applications (1993), Hindustan Antibiot. Bull., 35, 173-182.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | colorimetric or UV-determination of oxalic acid in biological fluids and beverages | Hordeum vulgare |
General Stability
| General Stability | Organism |
|---|---|
| immobilized on zirkonia coated alkylamine glass, retains 97% of enzyme activity | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | 1 M concentration 75% inhibition | Musa acuminata |  |
| Fe3+ | 10 mM concentration 35% inhibition | Musa acuminata | |
| NaCl | at 1 mM concentration 43% activity retained by free protein, 85% activity retained by immobilized protein | Hordeum vulgare | |
| Pb2+ | 2 mM concentration 57% inhibition, 20 mM concentration 89% inhibition | Musa acuminata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
oxalate | immobilized protein | Hordeum vulgare | |
| 0.42 | - |
oxalate | - |
Hordeum vulgare | |
| 1.88 | - |
oxalate | - |
Musa acuminata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Hordeum vulgare | 5829 | - |
| cytosol | - |
Musa acuminata | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
| Musa acuminata | - |
banana | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, under 3 months no loss of activity by free protein, over 2 years immobilized protein | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalic acid + O2 + 2 H+ | - |
Hordeum vulgare | 2 CO2 + H2O2 | - |
ir | |
| oxalic acid + O2 + 2 H+ | - |
Musa acuminata | 2 CO2 + H2O2 | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Hordeum vulgare |
| 60 | - |
70% activity retained | Musa acuminata |
| 75 | - |
80% wild type and 20% immobilized protein | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
- |
Hordeum vulgare |
| 4 | - |
immobilized protein | Hordeum vulgare |
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