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Literature summary for 1.2.3.3 extracted from
- Hydrogen peroxide-producing pyruvate oxidase from Lactobacillus delbrueckii is catalytically activated by phosphotidylethanolamine (2020), BMC Microbiol., 20, 128 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activating: phosphotidylcholine or phosphotidylglycerol | Lactobacillus delbrueckii | |
| phosphatidylethanolamine | enzyme is specifically activated by phosphotidylethanolamine (16:0-18:1). The C-terminal sequence is important for activation | Lactobacillus delbrueckii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.342 | - |
pyruvate | pH 5.6, 25°C | Lactobacillus delbrueckii | |
| 0.52 | - |
pyruvate | presence of phosphatidylethanolamine, pH 5.6, 25°C | Lactobacillus delbrueckii | |
| 1.25 | - |
phosphate | presence of phosphatidylethanolamine, pH 5.6, 25°C | Lactobacillus delbrueckii | |
| 8 | - |
phosphate | pH 5.6, 25°C | Lactobacillus delbrueckii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 345000 | - |
and 694000, gel filtration | Lactobacillus delbrueckii |
| 694000 | - |
and 345000, gel filtration | Lactobacillus delbrueckii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus delbrueckii | - |
- |
- |
| Lactobacillus delbrueckii STYM1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.32 | - |
pH 5.6, 25°C | Lactobacillus delbrueckii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + phosphate + O2 | - |
Lactobacillus delbrueckii | acetyl phosphate + CO2 + H2O2 | - |
? | |
| pyruvate + phosphate + O2 | - |
Lactobacillus delbrueckii STYM1 | acetyl phosphate + CO2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | and pentamer, 10 * 68000, SDS-PAGE | Lactobacillus delbrueckii |
| pentamer | and decamer, 5 * 68000, SDS-PAGE | Lactobacillus delbrueckii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POX | - |
Lactobacillus delbrueckii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
pyruvate | pH 5.6, 25°C | Lactobacillus delbrueckii | |
| 6.85 | - |
pyruvate | presence of phosphatidylethanolamine, pH 5.6, 25°C | Lactobacillus delbrueckii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Lactobacillus delbrueckii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Lactobacillus delbrueckii | |
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Release 2023.1 (January 2023)
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