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Literature summary for 1.2.3.15 extracted from

  • Son, Y.L.; Kim, H.Y.; Thiyagarajan, S.; Xu, J.J.; Park, S.M.
    Heterologous expression of Phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green (2012), Mycobiology, 40, 258-262.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + H2O + O2 Phanerodontia chrysosporium
-
methylglyoxylate + H2O2
-
?
methylglyoxal + H2O + O2 Phanerodontia chrysosporium BKM-F-1767
-
methylglyoxylate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q01772
-
-
Phanerodontia chrysosporium BKM-F-1767 Q01772
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Histag column chromatography, and gel filtration Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylglyoxal + H2O + O2
-
Phanerodontia chrysosporium methylglyoxylate + H2O2
-
?
methylglyoxal + H2O + O2
-
Phanerodontia chrysosporium BKM-F-1767 methylglyoxylate + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
GLX
-
Phanerodontia chrysosporium
Glx1
-
Phanerodontia chrysosporium
methylglyoxal oxidase
-
Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Phanerodontia chrysosporium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 about 35% activity at 20°C, 100% activity at 30°C, about 95% activity at 40°C, and about 40% activity at 50°C. Less than 20% activity between 60 and 80°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
4 6 more than 30% activity at pH 4.0 and 6.0, no activity at pH 3.0 and above pH 7.0 Phanerodontia chrysosporium