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Literature summary for 1.2.3.1 extracted from

  • Uchida, H.; Mikami, B.; Yamane-Tanabe, A.; Ito, A.; Hirano, K.; Oki, M.
    Crystal structure of an aldehyde oxidase from Methylobacillus sp. KY4400 (2018), J. Biochem., 163, 321-328 .
    View publication on PubMed
Search for more literature for 1.2.3.1

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of recombinant aldehyde oxidase at 2.5 A resolution, a narrow funnel leading to the si-face of the isoalloxazine ring of FAD is found. In the molybdenum catalytic centre a tryptophan is situated near the centre instead of the alanine present in other xanthine oxidase family members Methylobacillus sp. KY4400

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum protein contains 0.96 mol of Mo and 0.78 mol of 5'-CMP per mol of enzyme Methylobacillus sp. KY4400

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
142000
-
 gel filtration Methylobacillus sp. KY4400

Organism

Organism UniProt Comment Textmining
Methylobacillus sp. KY4400 Q84IX9 and Q84IY0 andQ84IX8 Q84IY0 i.e. small subunit AOMS, Q84IX9 i.e. medium subunit AOMM, Q84IX8 i.e. large subunit AOML
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + O2
-
 Methylobacillus sp. KY4400 acetic acid + H2O2
-
 ?
benzaldehyde + H2O + O2
-
 Methylobacillus sp. KY4400 benzoic acid + H2O2
-
 ?
butanal + H2O + O2
-
 Methylobacillus sp. KY4400 butyric acid + H2O2
-
 ?
formaldehyde + H2O + O2
-
 Methylobacillus sp. KY4400 formic acid + H2O2
-
 ?
heptanal + H2O + O2
-
 Methylobacillus sp. KY4400 heptanoic acid + H2O2
-
 ?
hexanal + H2O + O2
-
 Methylobacillus sp. KY4400 hexanoic acid + H2O2
-
 ?
additional information no substrates: xanthine and N'-methylnicotinamide Methylobacillus sp. KY4400 ?
-
-
vanillin + H2O + O2
-
 Methylobacillus sp. KY4400 vanillic acid + H2O2
-
 ?

Subunits

Subunits Comment Organism
trimer x * 17000 plus x * 35000 plus x * 83000, SDS-PAGE Methylobacillus sp. KY4400

Synonyms

Synonyms Comment Organism
AOMM
-
 Methylobacillus sp. KY4400

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center presence of a [4Fe-4 S] cluster in the medium subunit Methylobacillus sp. KY4400