Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.2.B2 extracted from

  • Grosse, S.; Voigt, C.; Wendlandt, K.D.; Kleber, H.P.
    Purification and properties of methanol dehydrogenase from Methylosinus sp. WI 14 (1998), J. Basic Microbiol., 8, 189-196.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Methylocystis sp.
140000
-
gel filtration Methylocystis sp.

Organism

Organism UniProt Comment Textmining
Methylocystis sp.
-
-
-
Methylocystis sp. WI14
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methylocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.12
-
-
Methylocystis sp.

Storage Stability

Storage Stability Organism
-18°C, solutions of the purified enzyme stored in 0.05 M sodium phosphate buffer, pH 7.5, show about 10% loss of activity during 24 h Methylocystis sp.
4°C, solutions of the purified enzyme stored in 0.05 M sodium phosphate buffer, pH 7.5, show about 30% loss of activity during 24 h Methylocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + oxidized 2,6-dichlorophenolindophenol
-
Methylocystis sp. acetate + reduced 2,6-dichlorophenolindophenol
-
?
acetaldehyde + oxidized 2,6-dichlorophenolindophenol
-
Methylocystis sp. WI14 acetate + reduced 2,6-dichlorophenolindophenol
-
?
formaldehyde + oxidized 2,6-dichlorophenolindophenol
-
Methylocystis sp. formate + reduced 2,6-dichlorophenolindophenol
-
?
formaldehyde + oxidized 2,6-dichlorophenolindophenol
-
Methylocystis sp. WI14 formate + reduced 2,6-dichlorophenolindophenol
-
?
additional information the enzyme from Methylosinus sp. WI 14 oxidizes only primary alcohols up to octanol and several aldehydes Methylocystis sp. ?
-
?
additional information the enzyme from Methylosinus sp. WI 14 oxidizes only primary alcohols up to octanol and several aldehydes Methylocystis sp. WI14 ?
-
?
propionaldehyde + oxidized 2,6-dichlorophenolindophenol
-
Methylocystis sp. propionate + reduced 2,6-dichlorophenolindophenol
-
?
propionaldehyde + oxidized 2,6-dichlorophenolindophenol
-
Methylocystis sp. WI14 propionate + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 70000, SDS-PAGE Methylocystis sp.

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone 0.65 mole per subunit Methylocystis sp.