Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Methylocystis sp. |
140000 | - |
gel filtration | Methylocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylocystis sp. | - |
- |
- |
Methylocystis sp. WI14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.12 | - |
- |
Methylocystis sp. |
Storage Stability | Organism |
---|---|
-18°C, solutions of the purified enzyme stored in 0.05 M sodium phosphate buffer, pH 7.5, show about 10% loss of activity during 24 h | Methylocystis sp. |
4°C, solutions of the purified enzyme stored in 0.05 M sodium phosphate buffer, pH 7.5, show about 30% loss of activity during 24 h | Methylocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + oxidized 2,6-dichlorophenolindophenol | - |
Methylocystis sp. | acetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
acetaldehyde + oxidized 2,6-dichlorophenolindophenol | - |
Methylocystis sp. WI14 | acetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
formaldehyde + oxidized 2,6-dichlorophenolindophenol | - |
Methylocystis sp. | formate + reduced 2,6-dichlorophenolindophenol | - |
? | |
formaldehyde + oxidized 2,6-dichlorophenolindophenol | - |
Methylocystis sp. WI14 | formate + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | the enzyme from Methylosinus sp. WI 14 oxidizes only primary alcohols up to octanol and several aldehydes | Methylocystis sp. | ? | - |
? | |
additional information | the enzyme from Methylosinus sp. WI 14 oxidizes only primary alcohols up to octanol and several aldehydes | Methylocystis sp. WI14 | ? | - |
? | |
propionaldehyde + oxidized 2,6-dichlorophenolindophenol | - |
Methylocystis sp. | propionate + reduced 2,6-dichlorophenolindophenol | - |
? | |
propionaldehyde + oxidized 2,6-dichlorophenolindophenol | - |
Methylocystis sp. WI14 | propionate + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 70000, SDS-PAGE | Methylocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | 0.65 mole per subunit | Methylocystis sp. |