BRENDA - Enzyme Database
show all sequences of 1.2.2.4

Cloning and molecular characterization of the genes for carbon monoxide dehydrogenase and localization of molybdopterin, flavin adenine dinucleotide, and iron-sulfur centers in the enzyme of Hydrogenophaga pseudoflava

Kang, B.S.; Kim, Y.M.; J. Bacteriol. 181, 5581-5590 (1999)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
genes for subunits L,M,S
Hydrogenophaga pseudoflava
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
[2Fe-2S] center
Hydrogenophaga pseudoflava
Molybdenum
-
Hydrogenophaga pseudoflava
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17750
-
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
30700
-
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
87200
-
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
Organism
Organism
UniProt
Commentary
Textmining
Hydrogenophaga pseudoflava
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
molybdoironflavoprotein
-
Hydrogenophaga pseudoflava
Subunits
Subunits
Commentary
Organism
?
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Hydrogenophaga pseudoflava
Cloned(Commentary) (protein specific)
Commentary
Organism
genes for subunits L,M,S
Hydrogenophaga pseudoflava
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Hydrogenophaga pseudoflava
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
[2Fe-2S] center
Hydrogenophaga pseudoflava
Molybdenum
-
Hydrogenophaga pseudoflava
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17750
-
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
30700
-
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
87200
-
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
molybdoironflavoprotein
-
Hydrogenophaga pseudoflava
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 87200, molybdopterin binding subunit L, + x * 30700, FAD-binding subunit M, + x * 17750, subunit S with two [2Fe-2S]-center binding sites, deduced from gene sequence
Hydrogenophaga pseudoflava
Other publictions for EC 1.2.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Swingley
The complete genome sequence o ...
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1
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1
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673552
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Three mammalian cytochromes b5 ...
Homo sapiens, Mus musculus, Rattus norvegicus
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1
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21
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3
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3
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21
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656481
Hanzelmann
The effect of intracellular mo ...
Hydrogenophaga pseudoflava
J. Mol. Biol.
301
1221-1235
2000
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2
1
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4
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2
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390482
Dobbek
Crystal structure and mechanis ...
Oligotropha carboxidovorans
Proc. Natl. Acad. Sci. USA
96
8884-8889
1999
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-
-
1
-
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3
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4
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1
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1
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1
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1
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3
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1
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1
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390483
Kang
Cloning and molecular characte ...
Hydrogenophaga pseudoflava
J. Bacteriol.
181
5581-5590
1999
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-
1
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2
3
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2
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1
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1
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1
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1
1
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2
3
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1
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1
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655594
Hanzelmann
-
The redox centers in the molyb ...
Pseudomonas thermocarboxydovorans
FEMS Microbiol. Lett.
176
139-145
1999
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-
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2
2
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1
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1
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1
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1
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1
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2
2
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1
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1
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11606
Jacobitz
Removal of CO dehydrogenase fr ...
Oligotropha carboxidovorans
J. Bacteriol.
171
6294-6299
1989
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-
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3
1
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4
-
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1
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1
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1
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11607
Meyer
-
Biochemistry, and physiology o ...
Hydrogenibacillus schlegelii, Hydrogenophaga pseudoflava, Oligotropha carboxidovorans, Pseudomonas carboxydohydrogena, Pseudomonas thermocarboxydovorans
FEMS Microbiol. Rev.
39
161-179
1986
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2
2
9
14
5
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5
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10
3
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8
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8
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2
2
9
14
5
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10
3
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