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Literature summary for 1.2.1.9 extracted from

  • Centeno-Leija, S.; Utrilla, J.; Flores, N.; Rodriguez, A.; Gosset, G.; Martinez, A.
    Metabolic and transcriptional response of Escherichia coli with a NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans (2013), Antonie van Leeuwenhoek, 104, 913-924 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gapN, construction of the mutant Escherichia coli strain MG1655DELTAgapA::gapN using the fusion of the gapN gene with a chloramphenicol resistance cassette resulting in plasmid pTrcgapN Streptococcus mutans

Protein Variants

Protein Variants Comment Organism
additional information the gapA gene, UniProt ID P0A9B2, EC 1.2.1.12, from Escherichia coli strain MG1655 is replaced by the gene gapN from Streptococcus mutans. The specific NADP+-GAPDH activity of the strain MG1655DgapA::gapN is 4.6times lower relative to strain MG1655DELTAgapA::gapN/pTrcgapN and no NAD+-GAPDH activity is detected. The specific NADP+-GAPDH activity levels in the derivative strain reveal that growth rate and glucose uptake differences are attributable to gapN expression level. The NADH/NAD+ ratio in the strain MG1655DELTAgapA::gapN/pTrcgapN decreases by 25% as compared to wild-type strain. In contrast, the NADPH/NADP+ ratio increases 2times indicating that the alteration in the turnover of NAD(P)H via glyceraldehyde 3-phosphate oxidation affects the redox levels of the strain MG1655DELTAgapA::gapN/pTrcgapN, which increases 2.8times the NADPH/NADH ratio Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O Streptococcus mutans
-
3-phospho-D-glycerate + NADPH + 2 H+
-
r

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q59931
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Streptococcus mutans 3-phospho-D-glycerate + NADPH + 2 H+
-
r

Synonyms

Synonyms Comment Organism
GAPN
-
Streptococcus mutans
NADP+-dependent GAPDH
-
Streptococcus mutans
NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase
-
Streptococcus mutans
NADP+-GAPDH
-
Streptococcus mutans

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Streptococcus mutans

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Streptococcus mutans
NADPH
-
Streptococcus mutans

General Information

General Information Comment Organism
metabolism the EMP pathway can be controlled through the glyceraldehyde 3-phosphate node by NAD+-GAPDH activity, recombinant NADP+-GAPDH heterologous activity can also exert a similar response, which modulates the glucose uptake and also the acetic acid production rate Streptococcus mutans