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Literature summary for 1.2.1.9 extracted from

  • Matsubara, K.; Yokooji, Y.; Atomi, H.; Imanaka, T.
    Biochemical and genetic characterization of the three metabolic routes in Thermococcus kodakarensis linking glyceraldehyde 3-phosphate and 3-phosphoglycerate (2011), Mol. Microbiol., 81, 1300-1312.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 1-phosphate
-
Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
D-glyceraldehyde 3-phosphate substrate inhibition Thermococcus kodakarensis
L-Glyceraldehyde 3-phosphate non-competitive inhibition Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55486
-
4 * 55486, calculated from amino acid sequence Thermococcus kodakarensis
230000
-
gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-glyceraldehyde 3-phosphate + NADP+ + H2O Thermococcus kodakarensis
-
3-phospho-DL-glycerate + NADPH + H+
-
?
additional information Thermococcus kodakarensis the enzyme does not react with formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, valeraldehyde, caproaldehyde, betaine aldehyde, glycolaldehyde, succinic semialdehyde or glyceraldehyde ?
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment (85┬░C, 30 min), anion exchange chromatography and gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Thermococcus kodakarensis 3-phospho-DL-glycerate + NADPH + H+
-
?
additional information the enzyme does not react with formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, valeraldehyde, caproaldehyde, betaine aldehyde, glycolaldehyde, succinic semialdehyde or glyceraldehyde Thermococcus kodakarensis ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 55486, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
GAPN
-
Thermococcus kodakarensis
non-phosphorylating GAP dehydrogenase
-
Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme activity is observed with both NAD+ and NADP+, but NADP+ is the preferred cofactor Thermococcus kodakarensis
NADP+ enzyme activity is observed with both NAD+ and NADP+, but NADP+ is the preferred cofactor Thermococcus kodakarensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
36.5
-
D-glyceraldehyde 3-phosphate in 100 mM MES/KOH (pH 7.0), at 70┬░C Thermococcus kodakarensis

General Information

General Information Comment Organism
malfunction DELTAgapN cells do not grow under glycolytic conditions Thermococcus kodakarensis
metabolism the enzyme plays a key role in providing NADPH under glycolytic conditions Thermococcus kodakarensis