Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose 1-phosphate | activates. Activation follows an increase in affinity for glyceraldehyde-3-phosphate rather than an increase in maximum velocity | Sulfurisphaera tokodaii | |
D-glucose 1-phosphate | activates. Activation follows an increase in maximum velocity rather than in affinity glyceraldehyde-3-phosphate | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
expression in Escherichia coli | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
E141D | kcat for D-glyceraldehyde 3-phosphate is 2.1fold lower than wild-type value | Sulfurisphaera tokodaii |
K137E | kcat for D-glyceraldehyde 3-phosphate is 1.1fold lower than wild-type value | Sulfurisphaera tokodaii |
L138T | kcat for D-glyceraldehyde 3-phosphate is 1.3fold lower than wild-type value | Sulfurisphaera tokodaii |
additional information | in non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus tokodaii, D-glucose 1-phosphate-induced activation follows an increase in affinity for glyceraldehyde-3-phosphate rather than an increase in maximum velocity, whereas in the enzyme from Sulfolobus solfataricus, D-glucose 1-phosphate-induced activation follows an increase in maximum velocity rather than in affinity for glyceraldehyde-3-phosphate. To explore the molecular basis of this difference 14 mutants and 2 chimeras are generated. The analyses of chimeric enzymes generated from regions of the Sulfolobus tokodaii enzyme and the Sulfolobus solfataricus enzyme indicates that a 57-residue module located in the subunit interface is involved in their allosteric behavior | Saccharolobus solfataricus |
additional information | in non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus tokodaii, D-glucose 1-phosphate-induced activation follows an increase in affinity for glyceraldehyde-3-phosphate rather than an increase in maximum velocity, whereas in the enzyme from Sulfolobus solfataricus, D-glucose 1-phosphate-induced activation follows an increase in maximum velocity rather than in affinity for glyceraldehyde-3-phosphate. To explore the molecular basis of this difference 14 mutants and 2 chimeras are generated. The analyses of chimeric enzymes generated from regions of the Sulfolobus tokodaii enzyme and the Sulfolobus solfataricus enzyme indicates that a 57-residue module located in the subunit interface is involved in their allosteric behavior | Sulfurisphaera tokodaii |
R136K | kcat for D-glyceraldehyde 3-phosphate is 2.8fold lower than wild-type value | Sulfurisphaera tokodaii |
Y139R | kcat for D-glyceraldehyde 3-phosphate is 302fold lower than wild-type value. The mutant enzyme no longer displays a sigmoidal K-type-like allostery but instead has apparent V-type allostery similar to that of the Sulfolobus solfataricus enzyme, suggesting that the residue located in the center of the homotetramer critically contributes to the allosteric behavior | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Sulfurisphaera tokodaii |
57000 | - |
4 * 57000, SDS-PAGE | Saccharolobus solfataricus |
223000 | - |
gel filtration | Sulfurisphaera tokodaii |
230000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97U30 | - |
- |
Saccharolobus solfataricus P2 | Q97U30 | - |
- |
Sulfurisphaera tokodaii | Q96XP0 | - |
- |
Sulfurisphaera tokodaii 7 | Q96XP0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
- |
Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Saccharolobus solfataricus | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Sulfurisphaera tokodaii | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? | |
D-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Sulfurisphaera tokodaii 7 | 3-phospho-D-glycerate + NADPH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 55000, SDS-PAGE | Sulfurisphaera tokodaii |
homotetramer | 4 * 57000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
GAPN | - |
Saccharolobus solfataricus |
GAPN | - |
Sulfurisphaera tokodaii |
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase | - |
Saccharolobus solfataricus |
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase | - |
Sulfurisphaera tokodaii |
SSO3194 | locus name | Saccharolobus solfataricus |
STK_24770 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
60 | - |
assay at | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C, mutant enzyme Y139R | Sulfurisphaera tokodaii | |
15 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C | Saccharolobus solfataricus | |
117 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C, mutant enzyme R136K | Sulfurisphaera tokodaii | |
161 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C, mutant enzyme E141D | Sulfurisphaera tokodaii | |
257 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C, mutant enzyme L138T | Sulfurisphaera tokodaii | |
297 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C, mutant enzyme K137E | Sulfurisphaera tokodaii | |
341 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 60°C, wild-type enzyme | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharolobus solfataricus |
8 | - |
assay at | Sulfurisphaera tokodaii |