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Literature summary for 1.2.1.9 extracted from

  • Iddar, A.; Valverde, F.; Assobhei, O.; Serrano, A.; Soukri, A.
    Widespread occurrence of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase among gram-positive bacteria (2005), Int. Microbiol., 8, 251-258.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
determined by SDS-PAGE and immunodetection Bacillus cereus
47000
-
determined by SDS-PAGE and immunodetection Bacillus thuringiensis
48000
-
determined by SDS-PAGE and immunodetection Clostridium perfringens
49000
-
determined by SDS-PAGE and immunodetection Clostridium acetobutylicum
52000
-
determined by SDS-PAGE and immunodetection Bacillus licheniformis
52000
-
determined by SDS-PAGE and immunodetection Streptococcus agalactiae
53000
-
determined by SDS-PAGE and immunodetection Clostridium sporogenes
53000
-
determined by SDS-PAGE and immunodetection Streptococcus sp.
55000
-
determined by SDS-PAGE and immunodetection Clostridioides difficile
55000
-
determined by SDS-PAGE and immunodetection Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O Bacillus sp. (in: Bacteria)
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Clostridium acetobutylicum
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Clostridium pasteurianum
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Clostridioides difficile
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Clostridium perfringens
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Clostridium sporogenes
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Bacillus cereus
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Bacillus licheniformis
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Bacillus thuringiensis
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Streptococcus sp.
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Streptococcus agalactiae
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Streptococcus pyogenes
-
3-phospho-D-glycerate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus cereus Q2HQS1
-
-
Bacillus licheniformis Q2HQS0
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus thuringiensis Q2HQR5
-
-
Clostridioides difficile Q2HQR4
-
-
Clostridium acetobutylicum Q2HQR9
-
-
Clostridium pasteurianum Q2HQR7
-
-
Clostridium perfringens Q2HQR8
-
-
Clostridium sporogenes Q2HQR6
-
-
Streptococcus agalactiae Q2HQS4
-
-
Streptococcus pyogenes Q2HQR3
-
-
Streptococcus sp. Q2HQS3
-
-

Purification (Commentary)

Purification (Comment) Organism
cell-free extract preparation Bacillus sp. (in: Bacteria)
cell-free extract preparation Clostridium acetobutylicum
cell-free extract preparation Clostridium pasteurianum
cell-free extract preparation Clostridioides difficile
cell-free extract preparation Clostridium perfringens
cell-free extract preparation Clostridium sporogenes
cell-free extract preparation Bacillus cereus
cell-free extract preparation Bacillus licheniformis
cell-free extract preparation Bacillus thuringiensis
cell-free extract preparation Streptococcus sp.
cell-free extract preparation Streptococcus agalactiae
cell-free extract preparation Streptococcus pyogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.01
-
-
Bacillus thuringiensis
0.011
-
-
Clostridium perfringens
0.013
-
-
Clostridium acetobutylicum
0.017
-
-
Streptococcus pyogenes
0.037
-
-
Clostridium sporogenes
0.038
-
-
Bacillus sp. (in: Bacteria)
0.04
-
-
Clostridioides difficile
0.04
-
-
Bacillus cereus
0.06
-
-
Clostridium pasteurianum
0.09
-
-
Streptococcus agalactiae
0.099
-
-
Bacillus licheniformis
0.102
-
-
Streptococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Bacillus sp. (in: Bacteria) 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Clostridium acetobutylicum 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Clostridium pasteurianum 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Clostridioides difficile 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Clostridium perfringens 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Clostridium sporogenes 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Bacillus cereus 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Bacillus licheniformis 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Bacillus thuringiensis 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Streptococcus sp. 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Streptococcus agalactiae 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Streptococcus pyogenes 3-phospho-D-glycerate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GAPDHN
-
Bacillus sp. (in: Bacteria)
GAPDHN
-
Clostridium acetobutylicum
GAPDHN
-
Clostridium pasteurianum
GAPDHN
-
Clostridioides difficile
GAPDHN
-
Clostridium perfringens
GAPDHN
-
Clostridium sporogenes
GAPDHN
-
Bacillus cereus
GAPDHN
-
Bacillus licheniformis
GAPDHN
-
Bacillus thuringiensis
GAPDHN
-
Streptococcus sp.
GAPDHN
-
Streptococcus agalactiae
GAPDHN
-
Streptococcus pyogenes
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Bacillus sp. (in: Bacteria)
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Clostridium acetobutylicum
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Clostridium pasteurianum
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Clostridioides difficile
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Clostridium perfringens
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Clostridium sporogenes
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Bacillus cereus
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Bacillus licheniformis
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Bacillus thuringiensis
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Streptococcus sp.
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Streptococcus agalactiae
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Streptococcus pyogenes
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Bacillus sp. (in: Bacteria)
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Clostridium acetobutylicum
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Clostridium pasteurianum
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Clostridioides difficile
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Clostridium perfringens
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Clostridium sporogenes
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Bacillus cereus
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Bacillus licheniformis
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Bacillus thuringiensis
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Streptococcus sp.
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Streptococcus agalactiae
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Streptococcus pyogenes

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
activity assay Bacillus sp. (in: Bacteria)
25
-
activity assay Clostridium acetobutylicum
25
-
activity assay Clostridium pasteurianum
25
-
activity assay Clostridioides difficile
25
-
activity assay Clostridium perfringens
25
-
activity assay Clostridium sporogenes
25
-
activity assay Bacillus cereus
25
-
activity assay Bacillus licheniformis
25
-
activity assay Bacillus thuringiensis
25
-
activity assay Streptococcus sp.
25
-
activity assay Streptococcus agalactiae
25
-
activity assay Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
activity assay Bacillus sp. (in: Bacteria)
8.5
-
activity assay Clostridium acetobutylicum
8.5
-
activity assay Clostridium pasteurianum
8.5
-
activity assay Clostridioides difficile
8.5
-
activity assay Clostridium perfringens
8.5
-
activity assay Clostridium sporogenes
8.5
-
activity assay Bacillus cereus
8.5
-
activity assay Bacillus licheniformis
8.5
-
activity assay Bacillus thuringiensis
8.5
-
activity assay Streptococcus sp.
8.5
-
activity assay Streptococcus agalactiae
8.5
-
activity assay Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Bacillus sp. (in: Bacteria)
NADPH
-
Clostridium acetobutylicum
NADPH
-
Clostridium pasteurianum
NADPH
-
Clostridioides difficile
NADPH
-
Clostridium perfringens
NADPH
-
Clostridium sporogenes
NADPH
-
Bacillus cereus
NADPH
-
Bacillus licheniformis
NADPH
-
Bacillus thuringiensis
NADPH
-
Streptococcus sp.
NADPH
-
Streptococcus agalactiae
NADPH
-
Streptococcus pyogenes