Literature summary for 1.2.1.9 extracted from

Iddar, A.; Valverde, F.; Assobhei, O.; Serrano, A.; Soukri, A.
Widespread occurrence of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase among gram-positive bacteria (2005), Int. Microbiol., 8, 251-258.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	determined by SDS-PAGE and immunodetection	Bacillus cereus
47000	-	determined by SDS-PAGE and immunodetection	Bacillus thuringiensis
48000	-	determined by SDS-PAGE and immunodetection	Clostridium perfringens
49000	-	determined by SDS-PAGE and immunodetection	Clostridium acetobutylicum
52000	-	determined by SDS-PAGE and immunodetection	Bacillus licheniformis
52000	-	determined by SDS-PAGE and immunodetection	Streptococcus agalactiae
53000	-	determined by SDS-PAGE and immunodetection	Clostridium sporogenes
53000	-	determined by SDS-PAGE and immunodetection	Streptococcus sp.
55000	-	determined by SDS-PAGE and immunodetection	Clostridioides difficile
55000	-	determined by SDS-PAGE and immunodetection	Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Bacillus sp. (in: Bacteria)	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Clostridium acetobutylicum	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Clostridium pasteurianum	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Clostridioides difficile	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Clostridium perfringens	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Clostridium sporogenes	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Bacillus cereus	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Bacillus licheniformis	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Bacillus thuringiensis	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Streptococcus sp.	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Streptococcus agalactiae	-	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	Streptococcus pyogenes	-	3-phospho-D-glycerate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	Q2HQS1	-	-
Bacillus licheniformis	Q2HQS0	-	-
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus thuringiensis	Q2HQR5	-	-
Clostridioides difficile	Q2HQR4	-	-
Clostridium acetobutylicum	Q2HQR9	-	-
Clostridium pasteurianum	Q2HQR7	-	-
Clostridium perfringens	Q2HQR8	-	-
Clostridium sporogenes	Q2HQR6	-	-
Streptococcus agalactiae	Q2HQS4	-	-
Streptococcus pyogenes	Q2HQR3	-	-
Streptococcus sp.	Q2HQS3	-	-

Purification (Commentary)

Purification (Comment)	Organism
cell-free extract preparation	Bacillus sp. (in: Bacteria)
cell-free extract preparation	Clostridium acetobutylicum
cell-free extract preparation	Clostridium pasteurianum
cell-free extract preparation	Clostridioides difficile
cell-free extract preparation	Clostridium perfringens
cell-free extract preparation	Clostridium sporogenes
cell-free extract preparation	Bacillus cereus
cell-free extract preparation	Bacillus licheniformis
cell-free extract preparation	Bacillus thuringiensis
cell-free extract preparation	Streptococcus sp.
cell-free extract preparation	Streptococcus agalactiae
cell-free extract preparation	Streptococcus pyogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.01	-	-	Bacillus thuringiensis
0.011	-	-	Clostridium perfringens
0.013	-	-	Clostridium acetobutylicum
0.017	-	-	Streptococcus pyogenes
0.037	-	-	Clostridium sporogenes
0.038	-	-	Bacillus sp. (in: Bacteria)
0.04	-	-	Clostridioides difficile
0.04	-	-	Bacillus cereus
0.06	-	-	Clostridium pasteurianum
0.09	-	-	Streptococcus agalactiae
0.099	-	-	Bacillus licheniformis
0.102	-	-	Streptococcus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Bacillus sp. (in: Bacteria)	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Clostridium acetobutylicum	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Clostridium pasteurianum	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Clostridioides difficile	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Clostridium perfringens	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Clostridium sporogenes	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Bacillus cereus	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Bacillus licheniformis	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Bacillus thuringiensis	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Streptococcus sp.	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Streptococcus agalactiae	3-phospho-D-glycerate + NADPH + H+	-	?
D-glyceraldehyde 3-phosphate + NADP+ + H2O	-	Streptococcus pyogenes	3-phospho-D-glycerate + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GAPDHN	-	Bacillus sp. (in: Bacteria)
GAPDHN	-	Clostridium acetobutylicum
GAPDHN	-	Clostridium pasteurianum
GAPDHN	-	Clostridioides difficile
GAPDHN	-	Clostridium perfringens
GAPDHN	-	Clostridium sporogenes
GAPDHN	-	Bacillus cereus
GAPDHN	-	Bacillus licheniformis
GAPDHN	-	Bacillus thuringiensis
GAPDHN	-	Streptococcus sp.
GAPDHN	-	Streptococcus agalactiae
GAPDHN	-	Streptococcus pyogenes
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Bacillus sp. (in: Bacteria)
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Clostridium acetobutylicum
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Clostridium pasteurianum
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Clostridioides difficile
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Clostridium perfringens
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Clostridium sporogenes
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Bacillus cereus
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Bacillus licheniformis
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Bacillus thuringiensis
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Streptococcus sp.
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Streptococcus agalactiae
glyceraldehyde-3-phosphate dehydrogenase (NADP+)	-	Streptococcus pyogenes
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Bacillus sp. (in: Bacteria)
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Clostridium acetobutylicum
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Clostridium pasteurianum
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Clostridioides difficile
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Clostridium perfringens
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Clostridium sporogenes
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Bacillus cereus
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Bacillus licheniformis
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Bacillus thuringiensis
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Streptococcus sp.
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Streptococcus agalactiae
non phosphorylating glyceraldehyde-3-phosphate dehydrogenase	-	Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay	Bacillus sp. (in: Bacteria)
25	-	activity assay	Clostridium acetobutylicum
25	-	activity assay	Clostridium pasteurianum
25	-	activity assay	Clostridioides difficile
25	-	activity assay	Clostridium perfringens
25	-	activity assay	Clostridium sporogenes
25	-	activity assay	Bacillus cereus
25	-	activity assay	Bacillus licheniformis
25	-	activity assay	Bacillus thuringiensis
25	-	activity assay	Streptococcus sp.
25	-	activity assay	Streptococcus agalactiae
25	-	activity assay	Streptococcus pyogenes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	activity assay	Bacillus sp. (in: Bacteria)
8.5	-	activity assay	Clostridium acetobutylicum
8.5	-	activity assay	Clostridium pasteurianum
8.5	-	activity assay	Clostridioides difficile
8.5	-	activity assay	Clostridium perfringens
8.5	-	activity assay	Clostridium sporogenes
8.5	-	activity assay	Bacillus cereus
8.5	-	activity assay	Bacillus licheniformis
8.5	-	activity assay	Bacillus thuringiensis
8.5	-	activity assay	Streptococcus sp.
8.5	-	activity assay	Streptococcus agalactiae
8.5	-	activity assay	Streptococcus pyogenes

Cofactor

Cofactor	Comment	Organism
NADPH	-	Bacillus sp. (in: Bacteria)
NADPH	-	Clostridium acetobutylicum
NADPH	-	Clostridium pasteurianum
NADPH	-	Clostridioides difficile
NADPH	-	Clostridium perfringens
NADPH	-	Clostridium sporogenes
NADPH	-	Bacillus cereus
NADPH	-	Bacillus licheniformis
NADPH	-	Bacillus thuringiensis
NADPH	-	Streptococcus sp.
NADPH	-	Streptococcus agalactiae
NADPH	-	Streptococcus pyogenes