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Literature summary for 1.2.1.9 extracted from

  • Asanuma, N.; Hino, T.
    Presence of NADP+-specific glyceraldehyde-3-phosphate dehydrogenase and CcpA-dependent transcription of its gene in the ruminal bacterium Streptococcus bovis (2006), FEMS Microbiol. Lett., 257, 17-23.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information gapN transcription is enhanced by the catabolite control protein A, CcpA Streptococcus equinus

Cloned(Commentary)

Cloned (Comment) Organism
EcoRI-digested genomic DNA into plasmid pUC18 for sequencing of gapN, into an expression vector for recombinant production of the enzyme in Escherichia coli Streptococcus equinus
for expression in Escherichia coli Streptococcus equinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51100
-
-
Streptococcus equinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O Streptococcus equinus
-
3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O Streptococcus equinus
-
3-phospho-D-glycerate + NADPH + H+
-
ir

Organism

Organism UniProt Comment Textmining
Streptococcus equinus Q3C1A6
-
-
Streptococcus equinus Q3C1A6 Streptococcus bovis gapN, sequence of gapN gene of S. mutans and S. pyogenes used for PCR primer design, Q59931, Q99Z67
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.001
-
growth stage: growth cessation, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.008
-
growth stage: just before the cessation of growth, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.011
-
growth stage: later log growth, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.014
-
strain 12U1-ccpA-, energy substrate glucose, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.014
-
strain 12U1-ccpA-, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.015
-
strain 12U1-ccpA-, energy substrate lactose, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.015
-
strain 12U1-ccpA-, energy substrate lactose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.016
-
strain 12U1, energy substrate lactose, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.016
-
strain 12U1, energy substrate lactose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.028
-
strain 12IU1p, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.028
-
strain 12U1p, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.032
-
growth stage: middle log growth, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.033
-
strain 12U1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.033
-
strain 12U1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.039
-
recombinant GAPN overexpression strain 12U1gapN-1, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.039
-
strain 12U1gapN-1, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus
0.065
-
recombinant GAPN overexpression strain 12U1gapN-2, energy substrate glucose, microM NADP+ reduced min-1 (mg of cellular nitrogen)-1 Streptococcus equinus
0.065
-
strain 12U1gapN-2, microM NADP+ reduced min-1 (mg of cell-N)-1 Streptococcus equinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Streptococcus equinus 3-phospho-D-glycerate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Streptococcus equinus 3-phospho-D-glycerate + NADPH + H+
-
ir

Synonyms

Synonyms Comment Organism
GAPN
-
Streptococcus equinus
glyceraldehyde-3-phosphate dehydrogenase
-
Streptococcus equinus
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
-
Streptococcus equinus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
activity assay at room temperature Streptococcus equinus
23
-
activity assay at room temperature Streptococcus equinus
60
-
-
Streptococcus equinus
60
-
optimal temperature for Streptococcus bovis GAPN Streptococcus equinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
activity assay Streptococcus equinus
8.5
-
optimal pH for Streptococcus bovis GAPN Streptococcus equinus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Streptococcus equinus

pI Value

Organism Comment pI Value Maximum pI Value
Streptococcus equinus
-
-
5.12