Protein Variants | Comment | Organism |
---|---|---|
R124L | turnover number is 21fold lower than turnover-number of wild-type enzym | Streptococcus mutans |
R301L | turnover number is 1000fold lower than turnover-number of wild-type enzyme. Rate limiting step is acylation, compared to deacylation in wild-type enzyme | Streptococcus mutans |
R459I | turnover number is 214fold lower than turnover-number of wild-type enzyme. Rate limiting step is acylation, compared to deacylation in wild-type enzyme | Streptococcus mutans |
Y170F | turnover number is 1.7fold higher than turnover-number of wild-type enzyme | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Glyceraldehyde-3-phosphate | - |
Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, wild-type enzyme | Streptococcus mutans | |
0.18 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, mutant enzyme Y170F | Streptococcus mutans | |
9.2 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, mutant enzyme R459I | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + NADP+ | rate-limiting step in wild-type enzyme and in mutant enzymes R124L, and Y170F is deacylation. Rate limiting step in mutant enzymes R301L and R459I is acylation | Streptococcus mutans | D-3-phosphoglycerate + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase | - |
Streptococcus mutans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, mutant enzyme R301L | Streptococcus mutans | |
0.28 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, mutant enzyme R459I | Streptococcus mutans | |
2.8 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, mutant enzyme R124L | Streptococcus mutans | |
60 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, wild-type enzyme | Streptococcus mutans | |
103 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C, mutant enzyme Y170F | Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptococcus mutans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
D-Glyceraldehyde-3-phosphate | pH 8.4, 25°C, wild-type enzyme | Streptococcus mutans | |
1.9 | - |
D-Glyceraldehyde-3-phosphate | pH 8.4, 25°C, mutant enzyme R459I | Streptococcus mutans |