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Literature summary for 1.2.1.9 extracted from

  • Marchal, S.; Branlant, G.
    Engineered nonphosphorylating gylceraldehyde 3-phosphate dehydrogenase at position 268 binds hydroxylamine and hydrazine as acyl acceptors (2001), Eur. J. Biochem., 268, 5764-5770.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E268A attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine Streptococcus mutans
E268Q attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
hydrazin protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism Streptococcus mutans
hydroxylamine protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+ Streptococcus mutans
-
D-3-phosphoglycerate + NADPH
-
ir

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-

Reaction

Reaction Comment Organism Reaction ID
D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ two-step mechanism and kinetics Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+
-
Streptococcus mutans D-3-phosphoglycerate + NADPH
-
ir
D-glyceraldehyde-3-phosphate + NADP+ two-step mechanism Streptococcus mutans D-3-phosphoglycerate + NADPH
-
ir

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Streptococcus mutans

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
steady-state rate constant for mutant E268Q and E268A is pH-dependent Streptococcus mutans

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Streptococcus mutans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.7
-
hydroxylamine wild-type enzyme Streptococcus mutans
6.3
-
hydrazin wild-type enzyme Streptococcus mutans
57
-
hydroxylamine mutant E268Q Streptococcus mutans
95
-
hydroxylamine mutant E268A Streptococcus mutans
109
-
hydrazin mutant E268A Streptococcus mutans
254
-
hydrazin mutant E268Q Streptococcus mutans