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Literature summary for 1.2.1.9 extracted from
- Enzymic and molecular characterization of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Synechoccocus PCC 7942: resistance of the enzyme to hydrogen peroxide (1996), Biochem. J., 316, 685-690.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21(DE3)-pLysS | Synechococcus sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
NADPH | 3-phosphoglycerate | Synechococcus sp. |  |
| 0.42 | - |
NADH | 3-phosphoglycerate | Synechococcus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
4 * 41000, SDS-PAGE | Synechococcus sp. |
| 160000 | - |
gel filtration | Synechococcus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus sp. | - |
not light-inducible | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| native and recombinant enzyme resistent to 1 mM H2O2 | Synechococcus sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Synechococcus sp. |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ | probably identical with EC 1.2.1.13 | Synechococcus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.9 | - |
purified enzyme, NADH | Synechococcus sp. |
| 150.5 | - |
purified native and recombinat enzyme, NADPH | Synechococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NADH + H+ | - |
Synechococcus sp. | D-glyceraldehyde 3-phosphate + NAD+ + H2O | - |
r | |
| D-glyceraldehyde-3-phosphate + NADP+ | - |
Synechococcus sp. | D-3-phosphoglycerate + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 41000, SDS-PAGE | Synechococcus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | low activity | Synechococcus sp. | |
| NADP+ | preference for | Synechococcus sp. | |
| NADPH | - |
Synechococcus sp. | |
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