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Literature summary for 1.2.1.9 extracted from

  • Tamoi, M.; Ishikawa, T.; Takeda, T.; Shigeoka, S.
    Enzymic and molecular characterization of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Synechoccocus PCC 7942: resistance of the enzyme to hydrogen peroxide (1996), Biochem. J., 316, 685-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3)-pLysS Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
NADPH 3-phosphoglycerate Synechococcus sp.
0.42
-
NADH 3-phosphoglycerate Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
4 * 41000, SDS-PAGE Synechococcus sp.
160000
-
gel filtration Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
not light-inducible
-

Oxidation Stability

Oxidation Stability Organism
native and recombinant enzyme resistent to 1 mM H2O2 Synechococcus sp.

Purification (Commentary)

Purification (Comment) Organism
-
Synechococcus sp.

Reaction

Reaction Comment Organism Reaction ID
D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ probably identical with EC 1.2.1.13 Synechococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
26.9
-
purified enzyme, NADH Synechococcus sp.
150.5
-
purified native and recombinat enzyme, NADPH Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NADH + H+
-
Synechococcus sp. D-glyceraldehyde 3-phosphate + NAD+ + H2O
-
r
D-glyceraldehyde-3-phosphate + NADP+
-
Synechococcus sp. D-3-phosphoglycerate + NADPH
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
NADH low activity Synechococcus sp.
NADP+ preference for Synechococcus sp.
NADPH
-
Synechococcus sp.