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Literature summary for 1.2.1.9 extracted from

  • Crow, V.L.; Wittenberger, C.L.
    Separation and properties of NAD+- and NADP+-dependent glyceraldehyde-3-phosphate dehydrogenases from Streptococcus mutans (1979), J. Biol. Chem., 254, 1134-1142.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3-phosphohydroxypyruvate
-
Streptococcus mutans
D-erythrose 4-phosphate
-
Streptococcus mutans
NADPH
-
Streptococcus mutans
sedoheptulose 7-phosphate
-
Streptococcus mutans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulation Streptococcus mutans
Na+ stimulation Streptococcus mutans
NH4+ stimulation Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
350000
-
-
Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+ Streptococcus mutans
-
D-3-phosphoglycerate + NADPH
-
ir

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus mutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
53
-
-
Streptococcus mutans

Storage Stability

Storage Stability Organism
-20Ā°C, 0.01 M potassium phosphate buffer, pH 6.4, 2 mM dithiothreitol, 50% v/v glycerol, 3 months Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+
-
Streptococcus mutans D-3-phosphoglycerate + NADPH
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.3
-
Streptococcus mutans

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.5 at pH 6.8: 19% of maximal activity, at pH 8.5: 92% of maximal activity Streptococcus mutans

Cofactor

Cofactor Comment Organism Structure
NADP+ absolutely specific for Streptococcus mutans