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Literature summary for 1.2.1.9 extracted from

  • Jacob, J.L.; D'Auzac, J.
    Glyceraldehyde-3-phosphate dehydrogenase from the latex of Hevea brasiliensis. Comparative study with its phosphorylating homologue (1972), Eur. J. Biochem., 31, 255-265.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP 3 mM, in coupled assay with aldolase Hevea brasiliensis
AMP 3 mM, in coupled assay with aldolase Hevea brasiliensis
ATP 3 mM, in coupled assay with aldolase Hevea brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Hevea brasiliensis
iodoacetamide
-
Hevea brasiliensis
Mg2+
-
Hevea brasiliensis
NADH
-
Hevea brasiliensis
phosphate
-
Hevea brasiliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADP+ coupled assay with aldolase Hevea brasiliensis
1.2
-
NAD+ glyceraldehyde 3-phosphate Hevea brasiliensis

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information aqueous phase of latex after centrifugation at 38000 x g Hevea brasiliensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000 200000 gel filtration Hevea brasiliensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+ Hevea brasiliensis
-
D-3-phosphoglycerate + NADPH
-
ir

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hevea brasiliensis

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
32.4
-
purified enzyme Hevea brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + NAD+ + H2O
-
Hevea brasiliensis 3-phospho-D-glycerate + NADH + H+
-
r
D-glyceraldehyde-3-phosphate + NADP+
-
Hevea brasiliensis D-3-phosphoglycerate + NADPH
-
ir

Subunits

Subunits Comment Organism
tetramer
-
Hevea brasiliensis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
6 min, 89% inactivation Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
Hevea brasiliensis

Cofactor

Cofactor Comment Organism Structure
NAD+ low activity Hevea brasiliensis
NADP+
-
Hevea brasiliensis