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Literature summary for 1.2.1.9 extracted from

  • Iglesias, A.A.; Serrano, A.; Guerrro, M.G.; Losada, M.
    Purification and properties of NADP-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the green algae Chlamydomonas reinhardtii (1987), Biochim. Biophys. Acta, 925, 1-10.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
-
Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
3-phosphohydroxypyruvate
-
Chlamydomonas reinhardtii
D-3-phosphoglycerate competitive Chlamydomonas reinhardtii
D-erythrose 4-phosphate
-
Chlamydomonas reinhardtii
iodoacetamide
-
Chlamydomonas reinhardtii
L-Glyceraldehyde 3-phosphate
-
Chlamydomonas reinhardtii
NADPH
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
NADP+
-
Chlamydomonas reinhardtii
0.051
-
D-glyceraldehyde 3-phosphate
-
Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000 200000 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+ Chlamydomonas reinhardtii
-
D-3-phosphoglycerate + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
8
-
-
Chlamydomonas reinhardtii

Storage Stability

Storage Stability Organism
4┬░C, 25 mM Tris-HCl buffer, pH 7.5, 0.1 mM EDTA, 10 mM mercaptoethanol, at least 1 month Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+
-
Chlamydomonas reinhardtii D-3-phosphoglycerate + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000-53000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
inactivation above Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Chlamydomonas reinhardtii