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Literature summary for 1.2.1.9 extracted from

  • Iglesias, A.A.; Losada, M.
    Purification and kinetic and structural properties of spinach leaf NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (1988), Arch. Biochem. Biophys., 260, 830-840.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-3-phosphoglycerate competitive Spinacia oleracea
Diamide
-
Spinacia oleracea
iodoacetamide
-
Spinacia oleracea
L-Glyceraldehyde 3-phosphate
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
NADP+
-
Spinacia oleracea
0.031
-
D-glyceraldehyde 3-phosphate
-
Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000 200000 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+ Spinacia oleracea may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm D-3-phosphoglycerate + NADPH
-
ir

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ proposed mechanism Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
15
-
-
Spinacia oleracea

Storage Stability

Storage Stability Organism
0-4Ā°C, 50 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 10 mM mercaptoethanol, at least 6 months, or precipitated in 60% ammonium sulfate Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+ absolutely specific for D-isomer Spinacia oleracea D-3-phosphoglycerate + NADPH
-
ir
D-glyceraldehyde-3-phosphate + NADP+ may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm Spinacia oleracea D-3-phosphoglycerate + NADPH
-
ir

Cofactor

Cofactor Comment Organism Structure
NADP+ absolutely specific for Spinacia oleracea