Cloned (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
expressed in Escherichia coli BL21(DE3) cells | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
34M/Y399C/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 55.7 | Thermoplasma acidophilum |
F34L | the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 3.2 | Thermoplasma acidophilum |
F34M/D372N/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 7.2 | Thermoplasma acidophilum |
F34M/D372N/Y399C/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 35.1 | Thermoplasma acidophilum |
F34M/S405N | enhances enzyme activity with the cofactor NAD+ by a factor of eight | Thermoplasma acidophilum |
F34M/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 8.5 while impacts on enzyme stability and substrate specificity are negligible | Thermoplasma acidophilum |
F34M/W271R/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 22.6 | Thermoplasma acidophilum |
F34M/W271S/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 42.5 | Thermoplasma acidophilum |
F34M/W271S/Y399C/S405N | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 1.1 | Thermoplasma acidophilum |
F34M/Y399C/S405N | enhances enzyme activity with the cofactor NAD+ by a factor of eight and enhances solubilty | Thermoplasma acidophilum |
S405C | the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 6 | Thermoplasma acidophilum |
S405N | the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 7.4 | Thermoplasma acidophilum |
W271S | the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 17.1 | Thermoplasma acidophilum |
Y399C | enhances the protein solubility after recombinant expression in Escherichia coli 6fold | Thermoplasma acidophilum |
Y399C | the mutation enhances the protein solubility after recombinant expression in Escherichia coli 6fold and enhances enzyme activity with the cofactor NAD+ by a factor of 6.4 | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADP+ | wild-type, pH 7.0, 50°C | Thermoplasma acidophilum | |
0.031 | - |
NADP+ | mutant F34M/Y399C/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
0.036 | - |
NADP+ | mutant F34M/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
16.7 | - |
NAD+ | mutant F34M/Y399C/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
16.7 | - |
NAD+ | mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C | Thermoplasma acidophilum | |
17.6 | - |
NAD+ | mutant F34M/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
17.6 | - |
NAD+ | mutant enzyme F34M/S405N, at pH 7.0 and 50°C | Thermoplasma acidophilum | |
22.7 | - |
NAD+ | wild-type, pH 7.0, 50°C | Thermoplasma acidophilum | |
22.7 | - |
NAD+ | wild type enzyme, at pH 7.0 and 50°C | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Thermoplasma acidophilum |
Organic Solvent | Comment | Organism |
---|---|---|
isobutanol | wild-type, 50% residual activity at 3.1%, mutant F34M/S405N at 3.6%, mutant F34M/Y399C/S405N at 3.5%, respectively | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HK01 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde + NAD+ | NAD+ is a very poor cofactor for wild-type | Thermoplasma acidophilum | D-glycerate + NADH + H+ | - |
? | |
D-glyceraldehyde + NADP+ + H2O | - |
Thermoplasma acidophilum | D-glycerate + NADPH + H+ | - |
? | |
glyceraldehyde + NAD+ + H2O | - |
Thermoplasma acidophilum | glycerate + NADH + H+ | - |
? | |
additional information | no activity with acetaldehyde and isobutyraldehyde | Thermoplasma acidophilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
ALDH | - |
Thermoplasma acidophilum |
Ta0809 | - |
Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.64 | - |
NAD+ | wild-type, pH 7.0, 50°C | Thermoplasma acidophilum | |
1.64 | - |
NAD+ | wild type enzyme, at pH 7.0 and 50°C | Thermoplasma acidophilum | |
10.54 | - |
NAD+ | mutant F34M/Y399C/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
10.54 | - |
NAD+ | mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C | Thermoplasma acidophilum | |
10.99 | - |
NAD+ | mutant F34M/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
10.99 | - |
NAD+ | mutant enzyme F34M/S405N, at pH 7.0 and 50°C | Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoplasma acidophilum | |
NADH | poor cofoactor | Thermoplasma acidophilum | |
NADPH | NADPH is preferred over NADH | Thermoplasma acidophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NAD+ | wild-type, pH 7.0, 50°C | Thermoplasma acidophilum | |
0.07 | - |
NAD+ | wild type enzyme, at pH 7.0 and 50°C | Thermoplasma acidophilum | |
0.62 | - |
NAD+ | mutant F34M/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
0.62 | - |
NAD+ | mutant enzyme F34M/S405N, at pH 7.0 and 50°C | Thermoplasma acidophilum | |
0.63 | - |
NAD+ | mutant F34M/Y399C/S405N, pH 7.0, 50°C | Thermoplasma acidophilum | |
0.63 | - |
NAD+ | mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C | Thermoplasma acidophilum |