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Literature summary for 1.2.1.89 extracted from
- Identification and characterization of Thermoplasma acidophilum glyceraldehyde dehydrogenase: a new class of NADP+-specific aldehyde dehydrogenase (2006), Biochem. J., 397, 131-138.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, activity is enhanced more than 2fold | Thermoplasma acidophilum |  |
| 4-chloromercuribenzoic acid | 1 mM, completely inhibits activity | Thermoplasma acidophilum | |
| 4-hydroxymercuribenzoic acid | 1 mM, completely inhibits activity | Thermoplasma acidophilum | |
| DTT | 1 mM, activity is enhanced more than 2fold | Thermoplasma acidophilum | |
| glutathione | 1 mM, activity is enhanced more than 2fold | Thermoplasma acidophilum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 12 h, 4°C, complete loss of activity | Thermoplasma acidophilum | |
| Cd2+ | 1 mM, 12 h, 4°C, 93% loss of activity | Thermoplasma acidophilum | |
| Cu2+ | 1 mM, 12 h, 4°C, complete loss of activity | Thermoplasma acidophilum | |
| Hg2+ | 1 mM, 12 h, 4°C, 99% loss of activity | Thermoplasma acidophilum | |
| Mg2+ | 1 mM, 12 h, 4°C, 24% loss of activity | Thermoplasma acidophilum | |
| Mn2+ | 1 mM, 12 h, 4°C, 44% loss of activity | Thermoplasma acidophilum | |
| Ni2+ | 1 mM, 12 h, 4°C, 58% loss of activity | Thermoplasma acidophilum | |
| Zn2+ | 1 mM, 12 h, 4°C, 46% loss of activity | Thermoplasma acidophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NADP+ | pH 8.0, 50°C | Thermoplasma acidophilum | |
| 0.33 | - |
D-glyceraldehyde | pH 8.0, 50°C | Thermoplasma acidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
4 * 53000, SDS-PAGE | Thermoplasma acidophilum |
| 54782 | - |
4 * 54782, calculated from sequence | Thermoplasma acidophilum |
| 215000 | - |
gel filtration | Thermoplasma acidophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde + NADP+ + H2O | Thermoplasma acidophilum | the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | Thermoplasma acidophilum JCM 9062 | the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway | D-glycerate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | Q9HK01 | - |
- |
| Thermoplasma acidophilum JCM 9062 | Q9HK01 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.85 | - |
pH 8.0, 50°C | Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde + NADP+ + H2O | the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway | Thermoplasma acidophilum | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | completely inactive when NAD+ is substituted for NADP+ | Thermoplasma acidophilum | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway | Thermoplasma acidophilum JCM 9062 | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde + NADP+ + H2O | completely inactive when NAD+ is substituted for NADP+ | Thermoplasma acidophilum JCM 9062 | D-glycerate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | activity is 33% compared to the activity with D-glyceraldehyde | Thermoplasma acidophilum | D-glycerate 3-phosphate + NADPH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + NADP+ + H2O | activity is 33% compared to the activity with D-glyceraldehyde | Thermoplasma acidophilum JCM 9062 | D-glycerate 3-phosphate + NADPH + H+ | - |
? | |
| glycolaldehyde + NADP+ | activity is 14% compared to the activity with D-glyceraldehyde | Thermoplasma acidophilum | glycolate + NADPH + H+ | - |
? | |
| glycolaldehyde + NADP+ | activity is 14% compared to the activity with D-glyceraldehyde | Thermoplasma acidophilum JCM 9062 | glycolate + NADPH + H+ | - |
? | |
| additional information | no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde) | Thermoplasma acidophilum | ? | - |
? | |
| additional information | no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde) | Thermoplasma acidophilum JCM 9062 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 53000, SDS-PAGE | Thermoplasma acidophilum |
| tetramer | 4 * 54782, calculated from sequence | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLD | - |
Thermoplasma acidophilum |
| glyceraldehyde dehydrogenase | - |
Thermoplasma acidophilum |
| Ta0809 | locus name | Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Thermoplasma acidophilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 50% of maximal activity, 70°C: about 60% of maximal activity | Thermoplasma acidophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 2 h | Thermoplasma acidophilum |
| 80 | - |
activity is decreased to less than 50% within 20 min | Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermoplasma acidophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 65% of maximal activity, pH 10.0: about 70% of maximal activity | Thermoplasma acidophilum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 24 h, stable | Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | completely inactive when NAD+ is substituted for NADP+ | Thermoplasma acidophilum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in downstream portion of the non-phosphorylated EntnerDoudoroff pathway | Thermoplasma acidophilum |
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