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Literature summary for 1.2.1.88 extracted from

  • Forlani, G.; Bertazzini, M.; Zarattini, M.; Funck, D.
    Functional characterization and expression analysis of rice delta(1)-pyrroline-5-carboxylate dehydrogenase provide new insight into the regulation of proline and arginine catabolism (2015), Front. Plant Sci., 6, 591.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.88

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Oryza sativa
Cu2+
-
 Oryza sativa
L-arginine uncompetitive Oryza sativa
Mn2+
-
 Oryza sativa
Zn2+
-
 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.265
-
 1-pyrroline-5-carboxylate cosubstrate NADP+, pH 7.5, 35°C Oryza sativa
0.358
-
 1-pyrroline-5-carboxylate cosubstrate NAD+, pH 7.5, 35°C Oryza sativa
0.644
-
 NAD+ pH 7.5, 35°C Oryza sativa
4.695
-
 NAD+ pH 7.5, 35°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58200
-
-
 Oryza sativa
259000
-
 gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Oryza sativa L-glutamate + NADH
-
 ?
1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Oryza sativa L-glutamate + NADPH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 58200, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
-
 Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47.5
-
 5 min, 50% loss of activity Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 NADP+ pH 7.5, 35°C Oryza sativa
12
-
 NAD+ pH 7.5, 35°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is preferred over NADP+ Oryza sativa
NADP+ NAD+ is preferred over NADP+ Oryza sativa

pI Value

Organism Comment pI Value Maximum pI Value
Oryza sativa isoelectric focusing
-
 5.6

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000022
-
 pH 7.5, 35°C Oryza sativa Cd2+
0.0015
-
 pH 7.5, 35°C Oryza sativa Zn2+
0.0024
-
 pH 7.5, 35°C Oryza sativa Mn2+
0.004
-
 pH 7.5, 35°C Oryza sativa Cu2+