Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.88 extracted from

  • Isobe, K.; Matsuzawa, T.; Soda, K.
    Crystallization and characterization of 1-pyrroline-5-carboxylate dehydrogenase from Bacillus sphaericus (1987), Agric. Biol. Chem., 51, 1947-1953.
No PubMed abstract available
Search for more literature for 1.2.1.88

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Lysinibacillus sphaericus

General Stability

General Stability Organism
NADP+ stabilizes Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0095
-
 NADP+
-
 Lysinibacillus sphaericus
0.42
-
 1-pyrroline-5-carboxylate with NADP+ Lysinibacillus sphaericus
0.75
-
 1-pyrroline-5-carboxylate with NAD+ Lysinibacillus sphaericus
2.3
-
 NAD+
-
 Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Lysinibacillus sphaericus
100000
-
 gel filtration Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 strains IFO 12983, ATCC 8005 and ATCC 21365
-
Geobacillus thermodenitrificans
-
 strains ATCC 39492 and DSM 465
-
Lysinibacillus sphaericus
-
-
-
[Bacillus] caldotenax
-
 strain DSM 406
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
 strain ATCC 21365 Geobacillus stearothermophilus
2.6
-
 strain ATCC 39492 Geobacillus thermodenitrificans
2.7
-
-
 [Bacillus] caldotenax
3.3
-
 strain DSM 465 Geobacillus thermodenitrificans
7.1
-
 strain ATCC 8005 Geobacillus stearothermophilus
8
-
 strain IFO 12983 Geobacillus stearothermophilus
34.1
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Geobacillus stearothermophilus L-glutamate + NADH
-
 ir
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 [Bacillus] caldotenax L-glutamate + NADH
-
 ir
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Geobacillus thermodenitrificans L-glutamate + NADH
-
 ir
1-pyrroline-5-carboxylate + NAD+ + H2O highly specific Lysinibacillus sphaericus L-glutamate + NADH
-
 ir
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Lysinibacillus sphaericus L-glutamate + NADH + H+
-
 r
1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Lysinibacillus sphaericus L-glutamate + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 cofactor NAD+ Lysinibacillus sphaericus
45
-
 cofactor NADP+ Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 15 min, 40% loss of activity in presence of NAD+, 2% loss of activity in presence of NADP+ Lysinibacillus sphaericus
40
-
 15 min, 75% loss of activity in presence of NAD+, 10% loss of activity in presence of NADP+ Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 cofactor NAD+ Lysinibacillus sphaericus
5.5 8 cofactor NADP+ Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Geobacillus stearothermophilus
NAD+
-
 Lysinibacillus sphaericus
NAD+
-
 [Bacillus] caldotenax
NAD+
-
 Geobacillus thermodenitrificans
NADP+ 8.1times higher activity compared to NAD+ Lysinibacillus sphaericus