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Literature summary for 1.2.1.85 extracted from

  • Inoue, J.; Shaw, J.P.; Rekik, M.; Harayama, S.
    Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida (1995), J. Bacteriol., 177, 1196-1201.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.85

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida
0.017
-
 2-hydroxymuconate-6-semialdehyde in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida
0.17
-
 3-Fluorobenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
0.33
-
 NAD+ in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
0.46
-
 benzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
0.53
-
 4-methylbenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
0.63
-
 3-methylbenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
0.81
-
 3-Nitrobenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
0.94
-
 3-Methoxybenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.3
-
 3-Chlorobenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
-
 Pseudomonas putida (2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+
-
 ?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas putida (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
 ?
3-chlorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-chlorobenzoate + NADH + H+
-
 ?
3-fluorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
3-methoxybenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methoxybenzoate + NADH + H+
-
 ?
3-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methylbenzoate + NADH + H+
-
 ?
3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-nitrobenzoate + NADH + H+
-
 ?
4-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-methylbenzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O weak substrate Pseudomonas putida benzoate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
2-hydroxymuconic semialdehyde dehydrogenase
-
 Pseudomonas putida
XylG
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 pH optimum for the oxidation of 2-hydroxymuconic semialdehyde Pseudomonas putida
9.6
-
 pH optimum for the oxidation of benzaldehyde Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 3-Methoxybenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
10
-
 4-methylbenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
20
-
 3-Nitrobenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
22
-
 3-Chlorobenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
32
-
 benzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
36
-
 3-methylbenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
56
-
 NAD+ apparent value, in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
330
-
 3-Fluorobenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1600
-
 2-hydroxymuconate-6-semialdehyde apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida
2100
-
 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida