Literature summary for 1.2.1.84 extracted from

Foo, J.L.; Rasouliha, B.H.; Susanto, A.V.; Leong, S.S.J.; Chang, M.W.
Engineering an alcohol-forming fatty acyl-CoA reductase for aldehyde and hydrocarbon biosynthesis in Saccharomyces cerevisiae (2020), Front. Bioeng. Biotechnol., 8, 585935 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae	Marinobacter nauticus

Protein Variants

Protein Variants	Comment	Organism
K156A	the mutant shows increased activity compared to the wild type enzyme	Marinobacter nauticus
K527A	inactive	Marinobacter nauticus
S126D	the mutant shows reduced activity compared to the wild type enzyme	Marinobacter nauticus
S126D/Y152F/K156A	the mutant shows increased activity compared to the wild type enzyme	Marinobacter nauticus
S515A	inactive	Marinobacter nauticus
Y152F	the mutant shows about wild type activity	Marinobacter nauticus
Y532F	inactive	Marinobacter nauticus

Organism

Organism	UniProt	Comment	Textmining
Marinobacter nauticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
amylose bead chromatography	Marinobacter nauticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0609	-	mutant enzyme S126D, at pH 7.0 and 25°C	Marinobacter nauticus
0.0632	-	wild type enzyme, at pH 7.0 and 25°C	Marinobacter nauticus
0.0641	-	mutant enzyme Y152F, at pH 7.0 and 25°C	Marinobacter nauticus
0.0702	-	mutant enzyme S126D/Y152F/K156A, at pH 7.0 and 25°C	Marinobacter nauticus
0.0732	-	mutant enzyme K156A, at pH 7.0 and 25°C	Marinobacter nauticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
palmitoyl-CoA + 2 NADPH + 2 H+	-	Marinobacter nauticus	hexadecanol + CoA + 2 NADP+	-	?

Synonyms

Synonyms	Comment	Organism
alcohol-forming fatty acyl-CoA reductase	-	Marinobacter nauticus
FACR	-	Marinobacter nauticus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Marinobacter nauticus

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Release 2023.1 (January 2023)