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Literature summary for 1.2.1.84 extracted from

  • Round, J.; Roccor, R.; Li, S.N.; Eltis, L.D.
    A fatty acyl coenzyme A reductase promotes wax ester accumulation in Rhodococcus jostii RHA1 (2017), Appl. Environ. Microbiol., 83, e00902-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.84

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography Rhodococcus jostii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0015
-
 with lauroyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
0.007
-
 with palmitoyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
0.041
-
 with oleoyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
0.045
-
 with stearoyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
2.8
-
 with cis-11-hexadecanal as substrate, at pH 7.0 and 25°C Rhodococcus jostii
3.3
-
 with decanal as substrate, at pH 7.0 and 25°C Rhodococcus jostii
5.3
-
 with dodecanal as substrate, at pH 7.0 and 25°C Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-11-hexadecenal + NADPH + H+
-
 Rhodococcus jostii cis-11-hexadecenol + NADP+
-
 ?
decanal + NADPH + H+
-
 Rhodococcus jostii decanol + NADP+
-
 ?
dodecanal + NADPH + H+
-
 Rhodococcus jostii dodecanol + NADP+
-
 ?
lauroyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii dodecanol + CoA + 2 NADP+
-
 ?
additional information no activity is detected when NADPH is substituted with NADH Rhodococcus jostii ?
-
-
oleoyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii octadecenol + CoA + 2 NADP+
-
 ?
palmitoyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii hexadecanol + CoA + 2 NADP+
-
 ?
stearoyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii stearyl alcohol + CoA + 2 NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 73627, calculated from amino acid sequence Rhodococcus jostii
? x * 75000, His6-tagged enzyme, SDS-PAGE Rhodococcus jostii

Synonyms

Synonyms Comment Organism
alcohol-forming fatty acyl-CoA reductase
-
 Rhodococcus jostii
fatty acyl coenzyme A reductase
-
 Rhodococcus jostii
FcrA
-
 Rhodococcus jostii
RS30405
-
 Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Rhodococcus jostii

General Information

General Information Comment Organism
physiological function the enzyme promotes wax ester accumulation in Rhodococcus jostii RHA1 Rhodococcus jostii