BRENDA - Enzyme Database
show all sequences of 1.2.1.81

Sulfoacetate is degraded via a novel pathway involving sulfoacetyl-CoA and sulfoacetaldehyde in Cupriavidus necator H16

Weinitschke, S.; Hollemeyer, K.; Kusian, B.; Bowien, B.; Smits, T.H.; Cook, A.M.; J. Biol. Chem. 285, 35249-35254 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Cupriavidus necator
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid
Cupriavidus necator
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.064
-
NADP+
pH 9.0, temperature not specified in the publication
Cupriavidus necator
0.102
-
CoA
pH 9.0, temperature not specified in the publication
Cupriavidus necator
0.33
-
2-sulfoacetaldehyde
pH 9.0, temperature not specified in the publication
Cupriavidus necator
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, SDS-PAGE
Cupriavidus necator
115000
-
gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfoacetyl-CoA + NADPH + H+
Cupriavidus necator
the enzyme is involved in degradation of sulfoacetate
2-sulfoacetaldehyde + CoA + NADP+
-
-
?
sulfoacetyl-CoA + NADPH + H+
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
the enzyme is involved in degradation of sulfoacetate
2-sulfoacetaldehyde + CoA + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
Q0K845
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Q0K845
-
-
Purification (Commentary)
Commentary
Organism
-
Cupriavidus necator
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:2-sulfoacetate-grown cell
inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction
Cupriavidus necator
-
Storage Stability
Storage Stability
Organism
4°C or -18°C, 1 week, 30% loss of activity
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-sulfoacetaldehyde + CoA + NADP+
specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid
709139
Cupriavidus necator
sulfoacetyl-CoA + NADPH + H+
-
-
-
r
2-sulfoacetaldehyde + CoA + NADP+
specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid
709139
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
sulfoacetyl-CoA + NADPH + H+
-
-
-
r
sulfoacetyl-CoA + NADPH + H+
the enzyme is involved in degradation of sulfoacetate
709139
Cupriavidus necator
2-sulfoacetaldehyde + CoA + NADP+
-
-
-
?
sulfoacetyl-CoA + NADPH + H+
the enzyme is involved in degradation of sulfoacetate
709139
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
2-sulfoacetaldehyde + CoA + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 52000, SDS-PAGE
Cupriavidus necator
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Cupriavidus necator
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
specific for NADP+, no activity with NAD+
Cupriavidus necator
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Cupriavidus necator
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
specific for NADP+, no activity with NAD+
Cupriavidus necator
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid
Cupriavidus necator
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.064
-
NADP+
pH 9.0, temperature not specified in the publication
Cupriavidus necator
0.102
-
CoA
pH 9.0, temperature not specified in the publication
Cupriavidus necator
0.33
-
2-sulfoacetaldehyde
pH 9.0, temperature not specified in the publication
Cupriavidus necator
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, SDS-PAGE
Cupriavidus necator
115000
-
gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfoacetyl-CoA + NADPH + H+
Cupriavidus necator
the enzyme is involved in degradation of sulfoacetate
2-sulfoacetaldehyde + CoA + NADP+
-
-
?
sulfoacetyl-CoA + NADPH + H+
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
the enzyme is involved in degradation of sulfoacetate
2-sulfoacetaldehyde + CoA + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cupriavidus necator
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:2-sulfoacetate-grown cell
inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction
Cupriavidus necator
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C or -18°C, 1 week, 30% loss of activity
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-sulfoacetaldehyde + CoA + NADP+
specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid
709139
Cupriavidus necator
sulfoacetyl-CoA + NADPH + H+
-
-
-
r
2-sulfoacetaldehyde + CoA + NADP+
specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid
709139
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
sulfoacetyl-CoA + NADPH + H+
-
-
-
r
sulfoacetyl-CoA + NADPH + H+
the enzyme is involved in degradation of sulfoacetate
709139
Cupriavidus necator
2-sulfoacetaldehyde + CoA + NADP+
-
-
-
?
sulfoacetyl-CoA + NADPH + H+
the enzyme is involved in degradation of sulfoacetate
709139
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
2-sulfoacetaldehyde + CoA + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 52000, SDS-PAGE
Cupriavidus necator
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Cupriavidus necator
Expression
Organism
Commentary
Expression
Cupriavidus necator
inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction
up
General Information
General Information
Commentary
Organism
malfunction
deletion mutant does not grow on sulfoacetate
Cupriavidus necator
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion mutant does not grow on sulfoacetate
Cupriavidus necator
Expression (protein specific)
Organism
Commentary
Expression
Cupriavidus necator
inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction
up
Other publictions for EC 1.2.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709139
Weinitschke
Sulfoacetate is degraded via a ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
J. Biol. Chem.
285
35249-35254
2010
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2
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1
4
1
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1
1
1
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