Cloned (Comment) | Organism |
---|---|
AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence expressed in Escherichia coli, both enzymes fused at the C-terminus to a His Tag containing maltose binding protein | Arabidopsis thaliana |
AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence subcloned into the pYES-DEST52 plasmid downstream of the GAL1 promoter and transformed into Saccharomyces cerevisiae W303-1A strain | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00148 | - |
hexadecanoyl-[acyl-carrier protein] | pH 7.0, at 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | transient expression of fusion proteins of AtFAR6 and yellow fluorescent protein in Nicotiana sp. leaves show that AtFAR6 is chloroplast-localized | Arabidopsis thaliana | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
in Escherichia coli expressed AtFAR6 and mAtFAR6 purified by a HisTrap HP column BioLogic LP liquid chromatography system | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | AtFAR6 promoter active in epidermis, endothecium and tapetum of anthers, histochemical staining of AtFAR6 promoter-GUS reporter gene activity | Arabidopsis thaliana | - |
additional information | no AtFAR6 promoter-GUS reporter gene activity in the inner cortex, vascular bundles or flower microspores | Arabidopsis thaliana | - |
plant epidermis | AtFAR6 promoter active in stem layer, histochemical staining of AtFAR6 promoter-GUS reporter gene activity | Arabidopsis thaliana | - |
receptacle | AtFAR6 promoter active in the replum and receptacle of siliques | Arabidopsis thaliana | - |
root cap | throughout lateral and primary root development, histochemical staining of AtFAR6 promoter-GUS reporter gene activity | Arabidopsis thaliana | - |
root primordium | histochemical staining of AtFAR6 promoter-GUS reporter gene activity | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ | acyl-CoA and acyl-ACP can serve as substrates | Arabidopsis thaliana | hexadecanal + acyl-carrier protein + NAD(P)+ | hexadecanal is a reaction intermediate that is further converted into hexadecanol | ? | |
additional information | in Saccharomyces cerevisiae overexpressed truncated version of AtFAR6, which lacks the N-terminal extension, produces C16:0 primary fatty alcohol as well as C18:0 fatty alcohols much more efficiently than the full length protein | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtFAR6 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | truncated mAtFAR6 protein | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant activity of truncated mAtFAR6 observed when NADH is used as cofactor | Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |