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Literature summary for 1.2.1.8 extracted from

  • Velasco-Garcia, R.; Gonzalez-Segura, L.; Munoz-Clares, R.A.
    Steady-state kinetic mechanism of the NADP+- and NAD+-dependent reactions catalysed by betaine aldehyde dehydrogenase from Pseudomonas aeruginosa (2000), Biochem. J., 352, 675-683.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
betaine product inhibition Pseudomonas aeruginosa
NADH product inhibition Pseudomonas aeruginosa
NADPH product inhibition Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 NAD+ 30°C, pH 8.0 Pseudomonas aeruginosa
0.385
-
 NADP+ 30°C, pH 8.0 Pseudomonas aeruginosa
0.434
-
 Betaine aldehyde 30°C, pH 8.0, cosubstrate NADP+ Pseudomonas aeruginosa
0.506
-
 Betaine aldehyde 30°C, pH 8.0, cosubstrate NAD+ Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ steady-state ordered mechanism, in which NAD+ binds first and NADH leaves last Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa betaine + NADH + H+
-
 ?
betaine aldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa betaine + NADPH + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
261
-
 NAD+ 30°C, pH 8.0 Pseudomonas aeruginosa
276
-
 NADP+ 30°C, pH 8.0 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas aeruginosa
NADP+
-
 Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.047
-
 NADH 30°C, pH 8.0 Pseudomonas aeruginosa
0.185
-
 NADPH 30°C, pH 8.0 Pseudomonas aeruginosa
0.209
-
 betaine 30°C, pH 8.0, cosubstrate NADP+ Pseudomonas aeruginosa
0.289
-
 betaine 30°C, pH 8.0, cosubstrate NAD+ Pseudomonas aeruginosa