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Literature summary for 1.2.1.8 extracted from

  • Incharoensakdi, A.; Matsuda, N.; Hibino, T.; Meng, Y.L.; Ishikawa, H.; Hara, A.; Funaguma, T.; Takabe, T.; Takabe, T.
    Overproduction of spinach betaine aldehyde dehydrogenase in Escherichia coli. Structural and functional properties of wild-type, mutants and E. coli enzymes (2000), Eur. J. Biochem., 267, 7015-7023.
    View publication on PubMed
Search for more literature for 1.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
of genes encoding five forms of spinach enzyme: full length, mature, mutant E103Q, mutant E103K and chimera Spinacia oleracea
overexpression in Escherichia coli Spinacia oleracea

Protein Variants

Protein Variants Comment Organism
E103K mutant enzyme has no activity with betaine aldehyde and 4-aminobutyraldehyde Spinacia oleracea
E103Q Km-values for 4-aminobutyraldehyde increases compared to wild-type Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 4-Aminobutyraldehyde wild-type enzyme Spinacia oleracea
0.0098
-
 4-Aminobutyraldehyde mutant enzyme E103Q Spinacia oleracea
0.023
-
 NAD+ mutant enzyme E103Q, reaction with betaine aldehyde Spinacia oleracea
0.024
-
 NAD+ mutant enzyme E103Q, reaction with 4-aminobutyraldehyde Spinacia oleracea
0.026
-
 NAD+ wild-type enzyme, reaction with 4-aminopropionaldehyde Spinacia oleracea
0.037
-
 4-Aminobutyraldehyde
-
 Spinacia oleracea
0.065
-
 Betaine aldehyde mutant enzyme E103Q Spinacia oleracea
0.068
-
 Betaine aldehyde wild-type enzyme Spinacia oleracea
0.07716
-
 Betaine aldehyde
-
 Spinacia oleracea
0.07799
-
 NAD+
-
 Spinacia oleracea
0.136
-
 NAD+ reaction with 4-aminobutyraldehyde Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutyraldehyde + NAD+ + H2O
-
 Spinacia oleracea 4-aminobutyrate + NADH
-
 ?
betaine aldehyde + NAD+ + H2O
-
 Spinacia oleracea betaine + NADH
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Spinacia oleracea