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Literature summary for 1.2.1.79 extracted from

  • Jang, E.H.; Park, S.A.; Chi, Y.M.; Lee, K.S.
    Kinetic and structural characterization for cofactor preference of succinic semialdehyde dehydrogenase from Streptococcus pyogenes (2014), Mol. Cells, 37, 719-726.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.79

Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
structures in apo-form and in a binary complex with NADP+ at 1.6 A and 2.1 A resolutions, respectively. Both structures show dimeric conformation and contain a single cysteine residue in the catalytic loop of each subunit. Residues Ser158 and Tyr188 participate in the stabilization of the 2'-phosphate group of adenine-side ribose in NADP+ Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NADP+ pH 7.0, 30°C Streptococcus pyogenes
0.23
-
 NAD+ pH 7.0, 30°C Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes A0A0J9X1M8
-
-
Streptococcus pyogenes MGAS1882 A0A0J9X1M8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
 Streptococcus pyogenes succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Streptococcus pyogenes MGAS1882 succinate + NADH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Streptococcus pyogenes succinate + NADPH + 2 H+
-
 ?
succinate semialdehyde + NADP+ + H2O
-
 Streptococcus pyogenes MGAS1882 succinate + NADPH + 2 H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.94
-
 NAD+ pH 7.0, 30°C Streptococcus pyogenes
7.2
-
 NADP+ pH 7.0, 30°C Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ is preferred over NAD+ Streptococcus pyogenes
NADP+ NADP+ is preferred over NAD+ Streptococcus pyogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.2
-
 NADP+ pH 7.0, 30°C Streptococcus pyogenes
9.7
-
 NAD+ pH 7.0, 30°C Streptococcus pyogenes