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Literature summary for 1.2.1.79 extracted from
- Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha (1999), FEMS Microbiol. Lett., 181, 63-71.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cupriavidus necator |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme does not obey Michaelis-Menten kinetics | Cupriavidus necator |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 5022 | - |
x * 5022, calculated, x * 51000, SDS-PAGE | Cupriavidus necator |
| 51000 | - |
x * 5022, calculated, x * 51000, SDS-PAGE | Cupriavidus necator |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | Q9RBF6 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.9 | - |
cosubstrate NAD+, pH 11.0, 30°C | Cupriavidus necator |
| 132.1 | - |
cosubstrate NADP+, pH 11.0, 30°C | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate semialdehyde + NAD+ + H2O | about 11% of the activity with NADP+ | Cupriavidus necator | succinate + NADH + 2 H+ | - |
? |  |
| succinate semialdehyde + NADP+ + H2O | - |
Cupriavidus necator | succinate + NADPH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 5022, calculated, x * 51000, SDS-PAGE | Cupriavidus necator |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 31 | - |
- |
Cupriavidus necator |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, stable | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | about 11% of the activity with NADP+ | Cupriavidus necator | |
| NADP+ | - |
Cupriavidus necator | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cupriavidus necator | the expression of gabD is not specifically induced by either 4-hydroxybutanoic acid or gamma-aminobutanoate | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | gene is disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky | Cupriavidus necator |
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