BRENDA - Enzyme Database
show all sequences of 1.2.1.78

Isolation and some properties of NAD-linked 2-carboxybenzaldehyde dehydrogenase in Alcaligenes faecalis AFK 2 grown on phenanthrene

Kiyohara, H.; Nagao, K.; Yano, K.; J. Gen. Appl. Microbiol. 27, 443-455 (1981)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-naphthoaldehyde
0.2 mM, 34.6% inhibition
Alcaligenes faecalis
4-carboxybenzaldehyde
0.2 mM, 38.5% inhibition
Alcaligenes faecalis
acetaldehyde
0.2 mM, 34.6% inhibition
Alcaligenes faecalis
Ag+
1 mM, complete inhibition
Alcaligenes faecalis
Co2+
1 mM, 40.2% inhibition
Alcaligenes faecalis
Cu2+
1 mM, complete inhibition
Alcaligenes faecalis
Hg2+
1 mM, 17.5% inhibition
Alcaligenes faecalis
iso-phthalaldehyde
0.2 mM, 23.8% inhibition
Alcaligenes faecalis
o-nitrobenzaldehyde
0.2 mM, 58.1% inhibition
Alcaligenes faecalis
o-phthalaldehyde
0.2 mM, 91.4% inhibition
Alcaligenes faecalis
p-chloromercuribenzoate
1 mM, complete inhibition
Alcaligenes faecalis
p-dimethylamino benzaldehyde
0.2 mM, 32.7% inhibition
Alcaligenes faecalis
propionaldehyde
0.2 mM, 31.7% inhibition
Alcaligenes faecalis
Protocatechualdehyde
0.2 mM, 23.1% inhibition
Alcaligenes faecalis
Salicylaldehyde
0.2 mM, 23.1% inhibition
Alcaligenes faecalis
SDS
1 mM, 44.3% inhibition
Alcaligenes faecalis
vertralaldehyde
0.2 mM, 13.5% inhibition
Alcaligenes faecalis
Zn2+
1 mM, 71.7% inhibition
Alcaligenes faecalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.054
-
2-carboxybenzaldehyde
pH 8.0, 30C
Alcaligenes faecalis
0.14
-
NAD+
pH 8.0, 30C
Alcaligenes faecalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
4 * 41000, SDS-PAGE
Alcaligenes faecalis
158000
-
gel filtration
Alcaligenes faecalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-carboxybenzaldehyde + NAD+ + H2O
Alcaligenes faecalis
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
o-phthalic acid + NADH + H+
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
Alcaligenes faecalis AFK2
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
o-phthalic acid + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis AFK2
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Alcaligenes faecalis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:phenanthrene-grown cell
-
Alcaligenes faecalis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
130.6
-
-
Alcaligenes faecalis
Storage Stability
Storage Stability
Organism
4C, activity in phosphate buffer is labile, 1 mM DTT stabilizes
Alcaligenes faecalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
699291
Alcaligenes faecalis
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor
699291
Alcaligenes faecalis
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
699291
Alcaligenes faecalis AFK2
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor
699291
Alcaligenes faecalis AFK2
o-phthalic acid + NADH + H+
-
-
-
?
additional information
no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde
699291
Alcaligenes faecalis
?
-
-
-
-
additional information
no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde
699291
Alcaligenes faecalis AFK2
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 41000, SDS-PAGE
Alcaligenes faecalis
Synonyms
Synonyms
Commentary
Organism
2CBAL dehydrogenase
-
Alcaligenes faecalis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Alcaligenes faecalis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
10 min, no loss of activity below 30C
Alcaligenes faecalis
40
-
10 min, 25% loss of activity
Alcaligenes faecalis
50
-
10 min, 94% loss of activity
Alcaligenes faecalis
60
-
10 min, complete loss of activity
Alcaligenes faecalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Alcaligenes faecalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8.6
pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity
Alcaligenes faecalis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
high specificity for NAD+ as electron acceptor
Alcaligenes faecalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
high specificity for NAD+ as electron acceptor
Alcaligenes faecalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-naphthoaldehyde
0.2 mM, 34.6% inhibition
Alcaligenes faecalis
4-carboxybenzaldehyde
0.2 mM, 38.5% inhibition
Alcaligenes faecalis
acetaldehyde
0.2 mM, 34.6% inhibition
Alcaligenes faecalis
Ag+
1 mM, complete inhibition
Alcaligenes faecalis
Co2+
1 mM, 40.2% inhibition
Alcaligenes faecalis
Cu2+
1 mM, complete inhibition
Alcaligenes faecalis
Hg2+
1 mM, 17.5% inhibition
Alcaligenes faecalis
iso-phthalaldehyde
0.2 mM, 23.8% inhibition
Alcaligenes faecalis
o-nitrobenzaldehyde
0.2 mM, 58.1% inhibition
Alcaligenes faecalis
o-phthalaldehyde
0.2 mM, 91.4% inhibition
Alcaligenes faecalis
p-chloromercuribenzoate
1 mM, complete inhibition
Alcaligenes faecalis
p-dimethylamino benzaldehyde
0.2 mM, 32.7% inhibition
Alcaligenes faecalis
propionaldehyde
0.2 mM, 31.7% inhibition
Alcaligenes faecalis
Protocatechualdehyde
0.2 mM, 23.1% inhibition
Alcaligenes faecalis
Salicylaldehyde
0.2 mM, 23.1% inhibition
Alcaligenes faecalis
SDS
1 mM, 44.3% inhibition
Alcaligenes faecalis
vertralaldehyde
0.2 mM, 13.5% inhibition
Alcaligenes faecalis
Zn2+
1 mM, 71.7% inhibition
Alcaligenes faecalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.054
-
2-carboxybenzaldehyde
pH 8.0, 30C
Alcaligenes faecalis
0.14
-
NAD+
pH 8.0, 30C
Alcaligenes faecalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
4 * 41000, SDS-PAGE
Alcaligenes faecalis
158000
-
gel filtration
Alcaligenes faecalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-carboxybenzaldehyde + NAD+ + H2O
Alcaligenes faecalis
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
o-phthalic acid + NADH + H+
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
Alcaligenes faecalis AFK2
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
o-phthalic acid + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Alcaligenes faecalis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:phenanthrene-grown cell
-
Alcaligenes faecalis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
130.6
-
-
Alcaligenes faecalis
Storage Stability (protein specific)
Storage Stability
Organism
4C, activity in phosphate buffer is labile, 1 mM DTT stabilizes
Alcaligenes faecalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
699291
Alcaligenes faecalis
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor
699291
Alcaligenes faecalis
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene
699291
Alcaligenes faecalis AFK2
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor
699291
Alcaligenes faecalis AFK2
o-phthalic acid + NADH + H+
-
-
-
?
additional information
no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde
699291
Alcaligenes faecalis
?
-
-
-
-
additional information
no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde
699291
Alcaligenes faecalis AFK2
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 41000, SDS-PAGE
Alcaligenes faecalis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Alcaligenes faecalis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
10 min, no loss of activity below 30C
Alcaligenes faecalis
40
-
10 min, 25% loss of activity
Alcaligenes faecalis
50
-
10 min, 94% loss of activity
Alcaligenes faecalis
60
-
10 min, complete loss of activity
Alcaligenes faecalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Alcaligenes faecalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8.6
pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity
Alcaligenes faecalis
Other publictions for EC 1.2.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639268
Iwabuchi
Biochemical and genetic charac ...
Nocardioides sp., Nocardioides sp. KP7
J. Bacteriol.
179
6488-6494
1997
-
-
1
-
-
-
6
3
-
-
2
2
-
2
-
-
1
-
-
1
-
-
8
1
1
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
3
-
-
2
2
-
-
-
1
-
1
-
-
8
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
699291
Kiyohara
-
Isolation and some properties ...
Alcaligenes faecalis, Alcaligenes faecalis AFK2
J. Gen. Appl. Microbiol.
27
443-455
1981
-
-
-
-
-
-
18
2
-
-
2
2
-
3
-
-
1
-
-
1
1
1
6
1
1
1
-
4
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
18
-
2
-
-
2
2
-
-
-
1
-
1
1
1
6
1
1
-
4
-
1
1
-
-
-
-
-
-
-
-