BRENDA - Enzyme Database
show all sequences of 1.2.1.78

Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7

Iwabuchi, T.; Harayama, S.; J. Bacteriol. 179, 6488-6494 (1997)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Nocardioides sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
10 mM, about 30% inhibition
Nocardioides sp.
EDTA
10 mM, 28% inhibition
Nocardioides sp.
ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid
10 mM, 14% inhibition
Nocardioides sp.
Mg2+
10 mM, about 30% inhibition
Nocardioides sp.
Mn2+
10 mM, about 30% inhibition
Nocardioides sp.
additional information
less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+
Nocardioides sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.083
-
NAD+
pH 9.4, 25°C
Nocardioides sp.
0.1
-
2-carboxybenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
2.8
-
2-carboxybenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
4 * 53000, SDS-PAGE
Nocardioides sp.
205000
-
gel filtration
Nocardioides sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-carboxybenzaldehyde + NAD+ + H2O
Nocardioides sp.
the enzyme is involved in phenanthrene degradation
o-phthalic acid + NADH + H+
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
Nocardioides sp. KP7
the enzyme is involved in phenanthrene degradation
o-phthalic acid + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nocardioides sp.
Q79EM7
-
-
Nocardioides sp. KP7
Q79EM7
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Nocardioides sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:phenanthrene-grown cell
-
Nocardioides sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-carboxybenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp.
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation
639268
Nocardioides sp.
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp. KP7
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation
639268
Nocardioides sp. KP7
o-phthalic acid + NADH + H+
-
-
-
?
2-nitrobenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp.
?
-
-
-
?
2-nitrobenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp. KP7
?
-
-
-
?
additional information
no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde
639268
Nocardioides sp.
?
-
-
-
-
additional information
no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde
639268
Nocardioides sp. KP7
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 53000, SDS-PAGE
Nocardioides sp.
Synonyms
Synonyms
Commentary
Organism
phdK
-
Nocardioides sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Nocardioides sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
32
-
NAD+
pH 9.4, 25°C
Nocardioides sp.
39
-
2-carboxybenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
48
-
2-nitrobenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Nocardioides sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Nocardioides sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Nocardioides sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Nocardioides sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
10 mM, about 30% inhibition
Nocardioides sp.
EDTA
10 mM, 28% inhibition
Nocardioides sp.
ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid
10 mM, 14% inhibition
Nocardioides sp.
Mg2+
10 mM, about 30% inhibition
Nocardioides sp.
Mn2+
10 mM, about 30% inhibition
Nocardioides sp.
additional information
less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+
Nocardioides sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.083
-
NAD+
pH 9.4, 25°C
Nocardioides sp.
0.1
-
2-carboxybenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
2.8
-
2-carboxybenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
4 * 53000, SDS-PAGE
Nocardioides sp.
205000
-
gel filtration
Nocardioides sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-carboxybenzaldehyde + NAD+ + H2O
Nocardioides sp.
the enzyme is involved in phenanthrene degradation
o-phthalic acid + NADH + H+
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
Nocardioides sp. KP7
the enzyme is involved in phenanthrene degradation
o-phthalic acid + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nocardioides sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:phenanthrene-grown cell
-
Nocardioides sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-carboxybenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp.
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation
639268
Nocardioides sp.
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp. KP7
o-phthalic acid + NADH + H+
-
-
-
?
2-carboxybenzaldehyde + NAD+ + H2O
the enzyme is involved in phenanthrene degradation
639268
Nocardioides sp. KP7
o-phthalic acid + NADH + H+
-
-
-
?
2-nitrobenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp.
?
-
-
-
?
2-nitrobenzaldehyde + NAD+ + H2O
-
639268
Nocardioides sp. KP7
?
-
-
-
?
additional information
no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde
639268
Nocardioides sp.
?
-
-
-
-
additional information
no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde
639268
Nocardioides sp. KP7
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 53000, SDS-PAGE
Nocardioides sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Nocardioides sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
32
-
NAD+
pH 9.4, 25°C
Nocardioides sp.
39
-
2-carboxybenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
48
-
2-nitrobenzaldehyde
pH 9.4, 25°C
Nocardioides sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Nocardioides sp.
Other publictions for EC 1.2.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639268
Iwabuchi
Biochemical and genetic charac ...
Nocardioides sp., Nocardioides sp. KP7
J. Bacteriol.
179
6488-6494
1997
-
-
1
-
-
-
6
3
-
-
2
2
-
2
-
-
1
-
-
1
-
-
8
1
1
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
3
-
-
2
2
-
-
-
1
-
1
-
-
8
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
699291
Kiyohara
-
Isolation and some properties ...
Alcaligenes faecalis, Alcaligenes faecalis AFK2
J. Gen. Appl. Microbiol.
27
443-455
1981
-
-
-
-
-
-
18
2
-
-
2
2
-
3
-
-
1
-
-
1
1
1
6
1
1
1
-
4
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
18
-
2
-
-
2
2
-
-
-
1
-
1
1
1
6
1
1
-
4
-
1
1
-
-
-
-
-
-
-
-