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Literature summary for 1.2.1.77 extracted from

  • Gescher, J.; Ismail, W.; Olgeschläger, E.; Eisenreich, W.; Wörth, J.; Fuchs, G.
    Aerobic benzoyl-coenzyme A (CoA) catabolic pathway in: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase (2006), J. Bacteriol., 188, 2919-2927.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a protein tagged at its N terminus with maltose-binding protein Aromatoleum evansii

Inhibitors

Inhibitors Comment Organism Structure
additional information salt concentrations up to 500 mM KCl and EDTA concentrations up to 50 mM have no effect on enzyme activity Aromatoleum evansii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NADP+ pH 7.2 Aromatoleum evansii
0.025
-
 3,4-dehydroadipyl-CoA semialdehyde pH 7.2 Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information salt concentrations up to 500 mM KCl have no effect on enzyme activity Aromatoleum evansii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000 Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O Aromatoleum evansii enzyme of the aerobic benzoyl-coenzyme A catabolic pathway 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aromatoleum evansii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 extracts of cells grown aerobically on benzoate Aromatoleum evansii

Storage Stability

Storage Stability Organism
-20°C, enzyme could be stored without appreciable loss of activity for months at -20°C in the presence of 10% (v/v) glycerol Aromatoleum evansii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O enzyme of the aerobic benzoyl-coenzyme A catabolic pathway Aromatoleum evansii 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O the molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1 Aromatoleum evansii 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 54000 Aromatoleum evansii

Synonyms

Synonyms Comment Organism
3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
-
 Aromatoleum evansii
BoxD
-
 Aromatoleum evansii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45
-
 NADP+ pH 7.2 Aromatoleum evansii
45
-
 3,4-dehydroadipyl-CoA semialdehyde pH 7.2 Aromatoleum evansii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Aromatoleum evansii

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.8 50% of maximal activity at pH 6.2 and pH 8.8 Aromatoleum evansii

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity (below 2%) with NAD+. The molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1 Aromatoleum evansii