BRENDA - Enzyme Database
show all sequences of 1.2.1.77

Structural and biochemical characterization of a novel aldehyde dehydrogenase encoded by the benzoate oxidation pathway in Burkholderia xenovorans LB400

Bajns J., Boulanger MJ.; J. Mol. Biol. 379, 597-608 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21 Star (DE3) cells
Paraburkholderia xenovorans
Crystallization (Commentary)
Crystallization (Commentary)
Organism
sitting drop vapour diffusion method, at 18C in 29% PEG 3350K and 100 mM bis-Tris (pH 6.0), 1.6 A crystal structure of ALDHC in complex with NADPH bound in the cofactor-binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel
Paraburkholderia xenovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADP+
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.042
-
heptaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.3
-
Valeraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.501
-
NAD+
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
1.21
-
propionaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
1.66
-
Isovaleraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
1.9
-
formaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
4.15
-
benzaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
Paraburkholderia xenovorans
the enzyme is involved in the benzoate oxidation (box) pathway
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Paraburkholderia xenovorans
Q13WK4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Paraburkholderia xenovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
the enzyme is involved in the benzoate oxidation (box) pathway
688414
Paraburkholderia xenovorans
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
-
?
benzaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
benzoate + NADPH + H+
-
-
-
?
formaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
formate + NADPH + H+
-
-
-
?
heptaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
heptanoate + NADPH + H+
-
-
-
?
isovaleraldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
isovalerate + NADPH + H+
-
-
-
?
additional information
the native substrate (3,4-dehydroadipyl-CoA semialdehyde) is not tested as it is commercially unavailable. The enzyme is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes
688414
Paraburkholderia xenovorans
?
-
-
-
-
propionaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
propionate + NADPH + H+
-
-
-
?
valeraldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
valerate + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
forms a dimer in solution with a molecular mass of 110000, SDS-PAGE
Paraburkholderia xenovorans
Synonyms
Synonyms
Commentary
Organism
ALDHC
-
Paraburkholderia xenovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.019
-
benzaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.04
-
NADP+
in 50 mM Tris-HCl (pH 7.5), at 25C, cosubstrate: propionaldehyde
Paraburkholderia xenovorans
0.046
-
formaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.074
-
Isovaleraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.501
-
NAD+
in 50 mM Tris-HCl (pH 7.5), at 25C, cosubstrate: propionaldehyde
Paraburkholderia xenovorans
2.32
-
propionaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
5.16
-
Valeraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
29.9
-
heptaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme
Paraburkholderia xenovorans
NADP+
the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme
Paraburkholderia xenovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 Star (DE3) cells
Paraburkholderia xenovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme
Paraburkholderia xenovorans
NADP+
the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme
Paraburkholderia xenovorans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapour diffusion method, at 18C in 29% PEG 3350K and 100 mM bis-Tris (pH 6.0), 1.6 A crystal structure of ALDHC in complex with NADPH bound in the cofactor-binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel
Paraburkholderia xenovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADP+
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.042
-
heptaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.3
-
Valeraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.501
-
NAD+
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
1.21
-
propionaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
1.66
-
Isovaleraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
1.9
-
formaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
4.15
-
benzaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
Paraburkholderia xenovorans
the enzyme is involved in the benzoate oxidation (box) pathway
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Paraburkholderia xenovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
the enzyme is involved in the benzoate oxidation (box) pathway
688414
Paraburkholderia xenovorans
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
-
?
benzaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
benzoate + NADPH + H+
-
-
-
?
formaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
formate + NADPH + H+
-
-
-
?
heptaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
heptanoate + NADPH + H+
-
-
-
?
isovaleraldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
isovalerate + NADPH + H+
-
-
-
?
additional information
the native substrate (3,4-dehydroadipyl-CoA semialdehyde) is not tested as it is commercially unavailable. The enzyme is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes
688414
Paraburkholderia xenovorans
?
-
-
-
-
propionaldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
propionate + NADPH + H+
-
-
-
?
valeraldehyde + NADP+ + H2O
-
688414
Paraburkholderia xenovorans
valerate + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
forms a dimer in solution with a molecular mass of 110000, SDS-PAGE
Paraburkholderia xenovorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.019
-
benzaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.04
-
NADP+
in 50 mM Tris-HCl (pH 7.5), at 25C, cosubstrate: propionaldehyde
Paraburkholderia xenovorans
0.046
-
formaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.074
-
Isovaleraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
0.501
-
NAD+
in 50 mM Tris-HCl (pH 7.5), at 25C, cosubstrate: propionaldehyde
Paraburkholderia xenovorans
2.32
-
propionaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
5.16
-
Valeraldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
29.9
-
heptaldehyde
in 50 mM Tris-HCl (pH 7.5), at 25C
Paraburkholderia xenovorans
Other publictions for EC 1.2.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726445
Bains
Elucidating the reaction mecha ...
Paraburkholderia xenovorans
Protein Sci.
20
1048-1059
2011
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1
1
8
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1
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1
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1
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1
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7
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1
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8
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7
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1
1
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712441
Rather
Coenzyme A-dependent aerobic m ...
Aromatoleum evansii, Aromatoleum evansii KB740
J. Biol. Chem.
285
20615-20624
2010
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1
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3
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1
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2
1
2
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1
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1
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1
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2
1
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688414
Bajns J.
Structural and biochemical cha ...
Paraburkholderia xenovorans
J. Mol. Biol.
379
597-608
2008
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1
1
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8
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1
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1
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1
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8
1
1
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8
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8
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8
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698572
Gescher
Aerobic benzoyl-coenzyme A (Co ...
Aromatoleum evansii
J. Bacteriol.
188
2919-2927
2006
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1
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1
2
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1
1
1
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4
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2
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