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Literature summary for 1.2.1.75 extracted from

  • Lee, J.; Cha, S.; Kang, C.; Lee, G.; Lim, H.; Jung, G.
    Efficient conversion of acetate to 3-hydroxypropionic acid by engineered Escherichia coli (2018), Catalysts, 8, 525 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene mcr, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of acetyl-CoA synthetase Chloroflexus aurantiacus

Protein Variants

Protein Variants Comment Organism
additional information for the efficient conversion of acetate to 3-hydroxypropionate(3-HP), heterologous mcr (encoding malonyl-CoA reductase) mutant N940V/K1106W/S1114R from Chloroflexus aurantiacus is initially introduced into Escherichia coli. Then, the acetate assimilating pathway and glyoxylate shunt pathway are activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, carbon flux to fatty acid synthesis is inhibited by adding cerulenin, which dramatically improves 3-HP production. Method evaluation and optimization, overview Chloroflexus aurantiacus
N940V/K1106W/S1114R site-directed mutagenesis, the mutant shows increased enzyme activity compared to wild-type enzyme Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + NADPH + H+ Chloroflexus aurantiacus
-
malonate semialdehyde + CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus Q6QQP7 bifunctional enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + NADPH + H+
-
Chloroflexus aurantiacus malonate semialdehyde + CoA + NADP+
-
?

Synonyms

Synonyms Comment Organism
MCR
-
Chloroflexus aurantiacus
More see also EC 1.1.1.298 Chloroflexus aurantiacus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Chloroflexus aurantiacus

General Information

General Information Comment Organism
metabolism the bifunctional enzyme from Chloroflexus aurantiacus synthesizes 3-hydroxypropionate (3-HP) from acetate via malonyl-CoA in the malonyl-CoA reductase pathway, enzyme MCR shows malonyl-CoA reductase activity, EC 1.1.1.298, and converts malonyl-CoA to malonate semialdehyde and CoA using NADPH. The malonate semialdehyde is then reduced to 3-hydroxypropionic acid, overview Chloroflexus aurantiacus