Literature summary for 1.2.1.75 extracted from
Lee, J.; Cha, S.; Kang, C.; Lee, G.; Lim, H.; Jung, G.
Efficient conversion of acetate to 3-hydroxypropionic acid by engineered Escherichia coli (2018), Catalysts, 8, 525 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene mcr, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of acetyl-CoA synthetase |
Chloroflexus aurantiacus |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
for the efficient conversion of acetate to 3-hydroxypropionate(3-HP), heterologous mcr (encoding malonyl-CoA reductase) mutant N940V/K1106W/S1114R from Chloroflexus aurantiacus is initially introduced into Escherichia coli. Then, the acetate assimilating pathway and glyoxylate shunt pathway are activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, carbon flux to fatty acid synthesis is inhibited by adding cerulenin, which dramatically improves 3-HP production. Method evaluation and optimization, overview |
Chloroflexus aurantiacus |
N940V/K1106W/S1114R |
site-directed mutagenesis, the mutant shows increased enzyme activity compared to wild-type enzyme |
Chloroflexus aurantiacus |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
malonyl-CoA + NADPH + H+ |
Chloroflexus aurantiacus |
- |
malonate semialdehyde + CoA + NADP+ |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Chloroflexus aurantiacus |
Q6QQP7 |
bifunctional enzyme |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
malonyl-CoA + NADPH + H+ |
- |
Chloroflexus aurantiacus |
malonate semialdehyde + CoA + NADP+ |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
MCR |
- |
Chloroflexus aurantiacus |
More |
see also EC 1.1.1.298 |
Chloroflexus aurantiacus |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
NADPH |
- |
Chloroflexus aurantiacus |
|
General Information
General Information |
Comment |
Organism |
metabolism |
the bifunctional enzyme from Chloroflexus aurantiacus synthesizes 3-hydroxypropionate (3-HP) from acetate via malonyl-CoA in the malonyl-CoA reductase pathway, enzyme MCR shows malonyl-CoA reductase activity, EC 1.1.1.298, and converts malonyl-CoA to malonate semialdehyde and CoA using NADPH. The malonate semialdehyde is then reduced to 3-hydroxypropionic acid, overview |
Chloroflexus aurantiacus |