BRENDA - Enzyme Database show
show all sequences of 1.2.1.73

Sulfoacetaldehyde is excreted quantitatively by Acinetobacter calcoaceticus SW1 during growth with taurine as sole source of nitrogen

Weinitschke, S.; von Rekowski, K.S.; Denger, K.; Cook, A.M.; Microbiology 151, 1285-1290 (2005)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
the enzyme is useful for determination of sulfoacetaldehyde concentrations
Rhodopseudomonas palustris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
2-sulfoacetaldehyde
pH 9.0, 22°C
Rhodopseudomonas palustris
0.092
-
NAD+
pH 9.0, 22°C
Rhodopseudomonas palustris
12
-
NADP+
pH 9.0, 22°C
Rhodopseudomonas palustris
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Rhodopseudomonas palustris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-sulfoacetaldehyde + H2O + NAD+
Rhodopseudomonas palustris
-
sulfoacetate + NADH + H+
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
Rhodopseudomonas palustris CGA009
-
sulfoacetate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodopseudomonas palustris
-
-
-
Rhodopseudomonas palustris CGA009
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
growth with taurine (2-aminoethanesulfonate) as the sole source of combined nitrogen under aerobic conditions
Rhodopseudomonas palustris
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7
-
purified enzyme
Rhodopseudomonas palustris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-sulfoacetaldehyde + H2O + NAD(P)+
NAD+ is the highly preferred cofactor
675824
Rhodopseudomonas palustris
sulfoacetate + NADH + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD(P)+
NAD+ is the highly preferred cofactor
675824
Rhodopseudomonas palustris CGA009
sulfoacetate + NADH + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
-
675824
Rhodopseudomonas palustris
sulfoacetate + NADH + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
-
675824
Rhodopseudomonas palustris CGA009
sulfoacetate + NADH + H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Rhodopseudomonas palustris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Rhodopseudomonas palustris
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
highly preferred cofactor
Rhodopseudomonas palustris
NADP+
low activity
Rhodopseudomonas palustris
Application (protein specific)
Application
Commentary
Organism
analysis
the enzyme is useful for determination of sulfoacetaldehyde concentrations
Rhodopseudomonas palustris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
highly preferred cofactor
Rhodopseudomonas palustris
NADP+
low activity
Rhodopseudomonas palustris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
2-sulfoacetaldehyde
pH 9.0, 22°C
Rhodopseudomonas palustris
0.092
-
NAD+
pH 9.0, 22°C
Rhodopseudomonas palustris
12
-
NADP+
pH 9.0, 22°C
Rhodopseudomonas palustris
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Rhodopseudomonas palustris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-sulfoacetaldehyde + H2O + NAD+
Rhodopseudomonas palustris
-
sulfoacetate + NADH + H+
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
Rhodopseudomonas palustris CGA009
-
sulfoacetate + NADH + H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
growth with taurine (2-aminoethanesulfonate) as the sole source of combined nitrogen under aerobic conditions
Rhodopseudomonas palustris
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7
-
purified enzyme
Rhodopseudomonas palustris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-sulfoacetaldehyde + H2O + NAD(P)+
NAD+ is the highly preferred cofactor
675824
Rhodopseudomonas palustris
sulfoacetate + NADH + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD(P)+
NAD+ is the highly preferred cofactor
675824
Rhodopseudomonas palustris CGA009
sulfoacetate + NADH + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
-
675824
Rhodopseudomonas palustris
sulfoacetate + NADH + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
-
675824
Rhodopseudomonas palustris CGA009
sulfoacetate + NADH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Rhodopseudomonas palustris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Rhodopseudomonas palustris
Other publictions for EC 1.2.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684762
Krejcik
Sulfoacetate released during t ...
Neptuniibacter caesariensis
Arch. Microbiol.
190
159-168
2008
-
-
1
-
-
1
1
3
1
1
4
2
-
3
-
-
1
-
-
-
-
-
3
1
1
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
1
-
1
-
3
1
1
4
2
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
675824
Weinitschke
Sulfoacetaldehyde is excreted ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009
Microbiology
151
1285-1290
2005
-
1
-
-
-
-
-
3
-
1
-
2
-
12
-
-
-
-
-
1
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
3
-
1
-
2
-
-
-
-
-
1
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684747
Denger
Sulfoacetate generated by Rhod ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009
Arch. Microbiol.
182
254-258
2004
-
-
-
-
-
-
-
-
-
1
-
2
-
9
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-