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Literature summary for 1.2.1.72 extracted from
- Biochemical characterization of gapB-encoded erythrose 4-phosphate dehydrogenase of Escherichia coli K-12 and its possible role in pyridoxal 5'-phosphate biosynthesis (1995), J. Bacteriol., 177, 2804-2812.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme requires the presence of a reducing agent, such as DTT or 2-mercaptoethanol, to maintain activity | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.074 | - |
NAD+ | - |
Escherichia coli |  |
| 0.96 | - |
D-erythrose 4-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37200 | - |
4 * 37200, SDS-PAGE | Escherichia coli |
| 132000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythrose 4-phosphate + NAD+ | Escherichia coli | the enzyme possible plays a role in the de novo biosynthesis of pyridoxal 5'-phosphate | 4-phosphoerythronate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for weeks with negligible loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythrose 4-phosphate + 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | 4-phosphoerythronate + ? | - |
? | |
| D-erythrose 4-phosphate + NAD+ | - |
Escherichia coli | 4-phosphoerythronate + NADH | - |
? | |
| D-erythrose 4-phosphate + NAD+ | the enzyme possible plays a role in the de novo biosynthesis of pyridoxal 5'-phosphate | Escherichia coli | 4-phosphoerythronate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 37200, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| E4PDH | - |
Escherichia coli |
| epd | - |
Escherichia coli |
| gap2 | renamed as epd | Escherichia coli |
| GapB | renamed as epd | Escherichia coli |
| gapB-encoded dehydrogenase | renamed as epd | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
at pH 8.6 | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 169 | - |
NAD+ | - |
Escherichia coli | |
| 200 | - |
D-erythrose 4-phosphate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | the pH profile rises steeply between pH 7 and 9 and then drops slightly at pH 10 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 3-acetylpyridine adenine dinucleotide | less efficient than NAD+ | Escherichia coli | |
| NAD+ | - |
Escherichia coli | |
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