BRENDA - Enzyme Database
show all sequences of 1.2.1.68

A coniferyl aldehyde dehydrogenase gene from Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by Saccharomyces cerevisiae

Adeboye, P.T.; Olsson, L.; Bettiga, M.; Biores. Technol. 212, 11-19 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
recombinant expression of the enzyme in Saccharomyces cerevisiae strain CEN.PK102-3A, deficient for acetaldehyde dehydrogenase Ald5 via deletion of ALD5, CALDH enzyme expression leads to increased coniferyl aldehyde conversion in the recombinant yeast strain. Effect of the conversion of coniferyl aldehyde on the lag phase of recombinant strains, overview
Pseudomonas sp. HR199
Engineering
Protein Variants
Commentary
Organism
additional information
improved endogenous conversion of phenolic compounds by heterologous expression of coniferyl aldehyde dehydrogenase enzyme from Pseudomonas in Saccharomyces cerevisiae, overview
Pseudomonas sp. HR199
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coniferaldehyde + CoA + NADP+
Pseudomonas sp. HR199
-
feruloyl-CoA + NADPH + H+
-
-
?
coniferaldehyde + CoA + NADP+
Pseudomonas sp. HR199 DSM 7063
-
feruloyl-CoA + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp. HR199
Q86447
-
-
Pseudomonas sp. HR199 DSM 7063
Q86447
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
the maximum specific growth rates of the strains in decreased by coniferaldehyde, no growth at 1.4 M coniferaldehyde
Pseudomonas sp. HR199
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
coniferaldehyde + CoA + NADP+
-
742102
Pseudomonas sp. HR199
feruloyl-CoA + NADPH + H+
-
-
-
?
coniferaldehyde + CoA + NADP+
-
742102
Pseudomonas sp. HR199 DSM 7063
feruloyl-CoA + NADPH + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CALDH
-
Pseudomonas sp. HR199
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas sp. HR199
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of the enzyme in Saccharomyces cerevisiae strain CEN.PK102-3A, deficient for acetaldehyde dehydrogenase Ald5 via deletion of ALD5, CALDH enzyme expression leads to increased coniferyl aldehyde conversion in the recombinant yeast strain. Effect of the conversion of coniferyl aldehyde on the lag phase of recombinant strains, overview
Pseudomonas sp. HR199
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas sp. HR199
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
improved endogenous conversion of phenolic compounds by heterologous expression of coniferyl aldehyde dehydrogenase enzyme from Pseudomonas in Saccharomyces cerevisiae, overview
Pseudomonas sp. HR199
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coniferaldehyde + CoA + NADP+
Pseudomonas sp. HR199
-
feruloyl-CoA + NADPH + H+
-
-
?
coniferaldehyde + CoA + NADP+
Pseudomonas sp. HR199 DSM 7063
-
feruloyl-CoA + NADPH + H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
the maximum specific growth rates of the strains in decreased by coniferaldehyde, no growth at 1.4 M coniferaldehyde
Pseudomonas sp. HR199
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
coniferaldehyde + CoA + NADP+
-
742102
Pseudomonas sp. HR199
feruloyl-CoA + NADPH + H+
-
-
-
?
coniferaldehyde + CoA + NADP+
-
742102
Pseudomonas sp. HR199 DSM 7063
feruloyl-CoA + NADPH + H+
-
-
-
?
Other publictions for EC 1.2.1.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742102
Adeboye
A coniferyl aldehyde dehydrog ...
Pseudomonas sp. HR199, Pseudomonas sp. HR199 DSM 7063
Biores. Technol.
212
11-19
2016
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654275
Overhage
Highly efficient biotransforma ...
Pseudomonas sp.
Appl. Environ. Microbiol.
69
6569-6576
2003
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1
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6
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654264
Overhage
Biotransformation of eugenol t ...
Pseudomonas sp.
Appl. Environ. Microbiol.
68
4315-4321
2002
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1
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3
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288325
Achterholt
Purification and characterizat ...
Pseudomonas sp.
J. Bacteriol.
180
4387-4391
1998
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1
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1
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