BRENDA - Enzyme Database
show all sequences of 1.2.1.68

Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16

Overhage, J.; Steinbuchel, A.; Priefert, H.; Appl. Environ. Microbiol. 68, 4315-4321 (2002)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. The gene calB, encoding coniferyl aldehyde dehydrogenase, and ehyAB and calA encoding eugenol hydroxylase and coniferyl alcohol dehydrogenase, respectively, are amplified and combined to construct a catabolic gene cassette. This gene cassette is cloned in the broad-host-range vector pBBR1-JO2 and transferred to Ralstonia eutropha H16. A recombinant strain of Ralstonia eutropha H16 harboring this plasmid expresses functionally active eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase. Cells of Ralstonia eutropha H16 from the late-exponential growth phase are used asa biocatalysts for the biotransformation of eugenol to ferulic acid. A maximum conversion rate of 2.9 mM of eugenol per h per liter of culture is achieved with a yield of 93.8 mol% of ferulic acid from eugenol within 20 h, without further optimization
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coniferyl aldehyde + H2O + NAD+
Pseudomonas sp.
involved in eugenol catabolism
ferulate + NADH
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
Q86447
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
coniferyl aldehyde + H2O + NAD+
-
654264
Pseudomonas sp.
ferulate + NADH
-
-
-
?
coniferyl aldehyde + H2O + NAD+
involved in eugenol catabolism
654264
Pseudomonas sp.
ferulate + NADH
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
coniferyl aldehyde dehydrogenase
-
Pseudomonas sp.
Application (protein specific)
Application
Commentary
Organism
biotechnology
biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. The gene calB, encoding coniferyl aldehyde dehydrogenase, and ehyAB and calA encoding eugenol hydroxylase and coniferyl alcohol dehydrogenase, respectively, are amplified and combined to construct a catabolic gene cassette. This gene cassette is cloned in the broad-host-range vector pBBR1-JO2 and transferred to Ralstonia eutropha H16. A recombinant strain of Ralstonia eutropha H16 harboring this plasmid expresses functionally active eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase. Cells of Ralstonia eutropha H16 from the late-exponential growth phase are used asa biocatalysts for the biotransformation of eugenol to ferulic acid. A maximum conversion rate of 2.9 mM of eugenol per h per liter of culture is achieved with a yield of 93.8 mol% of ferulic acid from eugenol within 20 h, without further optimization
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
coniferyl aldehyde + H2O + NAD+
Pseudomonas sp.
involved in eugenol catabolism
ferulate + NADH
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
coniferyl aldehyde + H2O + NAD+
-
654264
Pseudomonas sp.
ferulate + NADH
-
-
-
?
coniferyl aldehyde + H2O + NAD+
involved in eugenol catabolism
654264
Pseudomonas sp.
ferulate + NADH
-
-
-
?
Other publictions for EC 1.2.1.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742102
Adeboye
A coniferyl aldehyde dehydrog ...
Pseudomonas sp. HR199, Pseudomonas sp. HR199 DSM 7063
Biores. Technol.
212
11-19
2016
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654275
Overhage
Highly efficient biotransforma ...
Pseudomonas sp.
Appl. Environ. Microbiol.
69
6569-6576
2003
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654264
Overhage
Biotransformation of eugenol t ...
Pseudomonas sp.
Appl. Environ. Microbiol.
68
4315-4321
2002
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1
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288325
Achterholt
Purification and characterizat ...
Pseudomonas sp.
J. Bacteriol.
180
4387-4391
1998
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