BRENDA - Enzyme Database
show all sequences of 1.2.1.65

Metabolic regulation and chromosomal localization of carbaryl degradation pathway in Pseudomonas sp. strains C4, C5 and C6

Singh, R.; Trivedi, V.D.; Phale, P.S.; Arch. Microbiol. 195, 521-535 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
SALDH, DNA and amino acid sequence determination and analysis, the gene for SALDH is probably located on the chromosome in strains C4, C5 and C6, sequence comparisons and phylogenetic analysis
Pseudomonas sp. C6
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
salicylaldehyde + NAD+ + H2O
Pseudomonas sp. C6
-
salicylate + NADH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp. C6
I3QHK7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
Pseudomonas sp. strains C4, C5 and C6 utilize carbaryl as the sole source of carbon and energy via 1-naphthol, 1,2-dihydroxynaphthalene, benzalpyruvate, salicylaldehyde, salicylate, gentisate and maleylpyruvate, effects on enzyme activities of the carbaryl degradation pathway, overview. Cells grown on salicylate show poor activity for SALDH. Strains C4, C5 and C6 show low overall salicylaldehyde dehydrogenase activity
Pseudomonas sp. C6
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salicylaldehyde + NAD+ + H2O
-
741793
Pseudomonas sp. C6
salicylate + NADH + 2 H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas sp. C6
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp. C6
Cloned(Commentary) (protein specific)
Commentary
Organism
SALDH, DNA and amino acid sequence determination and analysis, the gene for SALDH is probably located on the chromosome in strains C4, C5 and C6, sequence comparisons and phylogenetic analysis
Pseudomonas sp. C6
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp. C6
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
salicylaldehyde + NAD+ + H2O
Pseudomonas sp. C6
-
salicylate + NADH + 2 H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
Pseudomonas sp. strains C4, C5 and C6 utilize carbaryl as the sole source of carbon and energy via 1-naphthol, 1,2-dihydroxynaphthalene, benzalpyruvate, salicylaldehyde, salicylate, gentisate and maleylpyruvate, effects on enzyme activities of the carbaryl degradation pathway, overview. Cells grown on salicylate show poor activity for SALDH. Strains C4, C5 and C6 show low overall salicylaldehyde dehydrogenase activity
Pseudomonas sp. C6
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salicylaldehyde + NAD+ + H2O
-
741793
Pseudomonas sp. C6
salicylate + NADH + 2 H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas sp. C6
General Information
General Information
Commentary
Organism
metabolism
pathway for the degradation of carbaryl via gentisate, overview. Various enzymes are involved in the metabolic pathway, including salicylaldehyde dehydroganse
Pseudomonas sp. C6
General Information (protein specific)
General Information
Commentary
Organism
metabolism
pathway for the degradation of carbaryl via gentisate, overview. Various enzymes are involved in the metabolic pathway, including salicylaldehyde dehydroganse
Pseudomonas sp. C6
Other publictions for EC 1.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743833
Jia
Evolutionary, computational, ...
Alteromonas naphthalenivorans
Sci. Rep.
7
43489
2017
-
-
1
-
4
-
1
9
-
-
1
1
-
4
-
-
1
1
-
-
-
-
2
2
-
1
-
8
1
1
-
1
4
-
-
-
-
1
1
-
4
-
-
1
4
9
-
-
1
1
-
-
-
1
-
-
-
-
2
2
-
1
-
8
1
1
-
-
1
5
5
1
8
8
741638
Singh
Purification and characteriza ...
Pseudomonas sp.
Appl. Biochem. Biotechnol.
172
806-819
2014
-
-
-
-
-
-
12
9
-
6
1
1
-
2
-
-
1
-
-
-
1
-
26
1
-
-
-
7
1
-
-
2
7
-
-
-
-
-
2
-
-
-
-
12
7
9
-
6
1
1
-
-
-
1
-
-
1
-
26
1
-
-
-
7
1
-
-
-
-
1
1
-
7
7
741793
Singh
Metabolic regulation and chro ...
Pseudomonas sp. C6
Arch. Microbiol.
195
521-535
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
723872
Coitinho
Expression, purification and p ...
Pseudomonas putida, Pseudomonas putida G7
Acta Crystallogr. Sect. F
68
93-97
2012
-
-
1
1
-
-
-
-
-
-
1
-
-
11
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725888
Li
Physiological role of the nove ...
Pseudomonas putida, Pseudomonas putida ND6
Microbiol. Res.
166
643-653
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
696505
Nayak
Metabolism of acenaphthylene v ...
Stenotrophomonas sp. RMSK
Biodegradation
20
837-843
2009
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
691830
Zhao
-
A novel salicylaldehyde dehydr ...
Pseudomonas putida, Pseudomonas putida ND6
Chin. Sci. Bull.
52
1942-1948
2007
-
1
1
-
-
-
8
4
-
4
1
-
-
4
-
-
1
-
-
-
-
-
28
1
-
-
1
-
-
-
-
1
-
2
-
-
2
2
2
-
-
-
-
15
-
4
-
5
2
-
-
-
-
2
-
-
-
-
28
2
-
-
2
-
-
-
-
2
-
-
-
-
-
-
288315
Manohar
Degradation of naphthalene by ...
Pseudomonas sp., Pseudomonas sp. NGK1
Indian J. Exp. Biol.
33
353-356
1995
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288316
Eaton
Bacterial metabolism of naphth ...
Pseudomonas putida, Pseudomonas putida PpG1064
J. Bacteriol.
174
7542-7554
1992
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288317
Connors
Metabolism of naphthalene by p ...
Pseudomonas sp.
J. Bacteriol.
141
1052-1054
1980
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288318
Austen
-
Regulation of the plasmid-spec ...
Pseudomonas putida, Pseudomonas putida PpG7
J. Gen. Microbiol.
117
521-528
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288319
Barnsley
Role and regulation of the ort ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Bacteriol.
125
404-408
1976
-
-
-
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288320
Barnsley
The induction of the enzymes o ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Gen. Microbiol.
88
193-196
1975
-
-
-
-
-
-
-
-
-
-
-
3
-
6
-
-
-
-
-
2
3
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288321
Shamsuzzaman
The regulation of naphthalene ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
60
582-589
1974
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-