BRENDA - Enzyme Database
show all sequences of 1.2.1.65

Physiological role of the novel salicylaldehyde dehydrogenase NahV in mineralization of naphthalene by Pseudomonas putida ND6

Li, S.; Li, X.; Zhao, H.; Cai, B.; Microbiol. Res. 166, 643-653 (2011)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ND6
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
15
-
nahF mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
Pseudomonas putida
67.81
-
nahV mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
Pseudomonas putida
85.4
-
wild-type ND6 with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
nahV mRNA levels are 3fold lower than the nahF mRNA levels in the wild-type ND6 strain
725888
Pseudomonas putida
?
-
-
-
?
additional information
nahV mRNA levels are 3fold lower than the nahF mRNA levels in the wild-type ND6 strain
725888
Pseudomonas putida ND6
?
-
-
-
?
salicylaldehyde + NAD+ + H2O
conversion of salicylaldehyde to salicylate in Pseudomonas putida ND6 is mediated by two isofunctional, inducible, plasmid-encoded enzymes, NahF and NahV
725888
Pseudomonas putida
salicylate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
conversion of salicylaldehyde to salicylate in Pseudomonas putida ND6 is mediated by two isofunctional, inducible, plasmid-encoded enzymes, NahF and NahV
725888
Pseudomonas putida ND6
salicylate + NADH + 2 H+
-
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
15
-
nahF mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
Pseudomonas putida
67.81
-
nahV mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
Pseudomonas putida
85.4
-
wild-type ND6 with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
nahV mRNA levels are 3fold lower than the nahF mRNA levels in the wild-type ND6 strain
725888
Pseudomonas putida
?
-
-
-
?
additional information
nahV mRNA levels are 3fold lower than the nahF mRNA levels in the wild-type ND6 strain
725888
Pseudomonas putida ND6
?
-
-
-
?
salicylaldehyde + NAD+ + H2O
conversion of salicylaldehyde to salicylate in Pseudomonas putida ND6 is mediated by two isofunctional, inducible, plasmid-encoded enzymes, NahF and NahV
725888
Pseudomonas putida
salicylate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
conversion of salicylaldehyde to salicylate in Pseudomonas putida ND6 is mediated by two isofunctional, inducible, plasmid-encoded enzymes, NahF and NahV
725888
Pseudomonas putida ND6
salicylate + NADH + 2 H+
-
-
-
?
Expression
Organism
Commentary
Expression
Pseudomonas putida
a 10fold increase in nahV expression observed in the ND6-NahF mutant when cultured in the presence of naphthalene and a 30fold increase when cultured in the presence of salicylate; nahF expression levels in the ND6-NahV mutant elevated by 20fold compared to the levels observed in the wild-type strain cultured in the presence of naphthalene and a 5fold increase when cultured in the presence of salicylate
up
General Information
General Information
Commentary
Organism
metabolism
mineralization of naphthalene, detoxification of salicylaldehyde
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
metabolism
mineralization of naphthalene, detoxification of salicylaldehyde
Pseudomonas putida
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas putida
a 10fold increase in nahV expression observed in the ND6-NahF mutant when cultured in the presence of naphthalene and a 30fold increase when cultured in the presence of salicylate; nahF expression levels in the ND6-NahV mutant elevated by 20fold compared to the levels observed in the wild-type strain cultured in the presence of naphthalene and a 5fold increase when cultured in the presence of salicylate
up
Other publictions for EC 1.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743833
Jia
Evolutionary, computational, ...
Alteromonas naphthalenivorans
Sci. Rep.
7
43489
2017
-
-
1
-
4
-
1
9
-
-
1
1
-
4
-
-
1
1
-
-
-
-
2
2
-
1
-
8
1
1
-
1
4
-
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1
1
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4
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1
4
9
-
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1
1
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-
1
-
-
-
-
2
2
-
1
-
8
1
1
-
-
1
5
5
1
8
8
741638
Singh
Purification and characteriza ...
Pseudomonas sp.
Appl. Biochem. Biotechnol.
172
806-819
2014
-
-
-
-
-
-
12
9
-
6
1
1
-
2
-
-
1
-
-
-
1
-
26
1
-
-
-
7
1
-
-
2
7
-
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-
-
2
-
-
-
-
12
7
9
-
6
1
1
-
-
-
1
-
-
1
-
26
1
-
-
-
7
1
-
-
-
-
1
1
-
7
7
741793
Singh
Metabolic regulation and chro ...
Pseudomonas sp. C6
Arch. Microbiol.
195
521-535
2013
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-
1
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1
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2
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1
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1
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1
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1
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1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
723872
Coitinho
Expression, purification and p ...
Pseudomonas putida, Pseudomonas putida G7
Acta Crystallogr. Sect. F
68
93-97
2012
-
-
1
1
-
-
-
-
-
-
1
-
-
11
-
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1
-
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-
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2
1
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1
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1
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1
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1
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2
1
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-
-
-
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-
1
1
-
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-
725888
Li
Physiological role of the nove ...
Pseudomonas putida, Pseudomonas putida ND6
Microbiol. Res.
166
643-653
2011
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-
-
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-
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7
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3
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4
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3
-
4
-
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-
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-
1
1
1
1
-
-
696505
Nayak
Metabolism of acenaphthylene v ...
Stenotrophomonas sp. RMSK
Biodegradation
20
837-843
2009
-
1
-
-
-
-
-
-
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1
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2
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1
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2
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2
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1
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1
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1
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1
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1
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1
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2
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2
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1
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691830
Zhao
-
A novel salicylaldehyde dehydr ...
Pseudomonas putida, Pseudomonas putida ND6
Chin. Sci. Bull.
52
1942-1948
2007
-
1
1
-
-
-
8
4
-
4
1
-
-
4
-
-
1
-
-
-
-
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28
1
-
-
1
-
-
-
-
1
-
2
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-
2
2
2
-
-
-
-
15
-
4
-
5
2
-
-
-
-
2
-
-
-
-
28
2
-
-
2
-
-
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-
2
-
-
-
-
-
-
288315
Manohar
Degradation of naphthalene by ...
Pseudomonas sp., Pseudomonas sp. NGK1
Indian J. Exp. Biol.
33
353-356
1995
-
-
-
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-
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2
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2
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1
1
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2
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1
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1
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2
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1
1
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2
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288316
Eaton
Bacterial metabolism of naphth ...
Pseudomonas putida, Pseudomonas putida PpG1064
J. Bacteriol.
174
7542-7554
1992
-
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1
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2
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6
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1
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2
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1
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1
1
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2
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1
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2
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288317
Connors
Metabolism of naphthalene by p ...
Pseudomonas sp.
J. Bacteriol.
141
1052-1054
1980
-
-
1
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-
-
-
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1
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1
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1
2
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1
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1
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1
1
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1
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1
2
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1
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288318
Austen
-
Regulation of the plasmid-spec ...
Pseudomonas putida, Pseudomonas putida PpG7
J. Gen. Microbiol.
117
521-528
1980
-
-
-
-
-
-
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2
-
5
-
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1
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2
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1
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1
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2
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1
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2
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-
288319
Barnsley
Role and regulation of the ort ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Bacteriol.
125
404-408
1976
-
-
-
-
-
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3
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6
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2
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3
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2
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2
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3
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2
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3
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288320
Barnsley
The induction of the enzymes o ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Gen. Microbiol.
88
193-196
1975
-
-
-
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3
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6
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2
3
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3
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2
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2
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3
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2
3
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3
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288321
Shamsuzzaman
The regulation of naphthalene ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
60
582-589
1974
-
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-
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1
1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
1
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1
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1
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