BRENDA - Enzyme Database
show all sequences of 1.2.1.65

Expression, purification and preliminary crystallographic studies of NahF, a salicylaldehyde dehydrogenase from Pseudomonas putida G7 involved in naphthalene degradation

Coitinho, J.B.; Costa, D.M.; Guimaraes, S.L.; de Goes, A.M.; Nagem, R.A.; Acta Crystallogr. Sect. F 68, 93-97 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
nahF gene subcloned into the pET28a(TEV) vector and the recombinant protein with 6xHis tag at the N-terminaus overexpressed in Escherichia coli BL21 Arctic Express (DE3) cells
Pseudomonas putida
Crystallization (Commentary)
Crystallization
Organism
crystals of recombinant 6xHis-NahF obtained at 291 K and diffracted to 2.42 A resolution
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
125000
-
-
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida G7
-
-
-
Purification (Commentary)
Commentary
Organism
by Ni2+ affinity chromatography followed by gel filtration
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase
723872
Pseudomonas putida
?
-
-
-
?
additional information
nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase
723872
Pseudomonas putida G7
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
suggested by the elution profile in the SEC chromatogram
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
nahF gene subcloned into the pET28a(TEV) vector and the recombinant protein with 6xHis tag at the N-terminaus overexpressed in Escherichia coli BL21 Arctic Express (DE3) cells
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystals of recombinant 6xHis-NahF obtained at 291 K and diffracted to 2.42 A resolution
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
125000
-
-
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
by Ni2+ affinity chromatography followed by gel filtration
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase
723872
Pseudomonas putida
?
-
-
-
?
additional information
nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase
723872
Pseudomonas putida G7
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
suggested by the elution profile in the SEC chromatogram
Pseudomonas putida
General Information
General Information
Commentary
Organism
metabolism
NahF catalyzes the last reaction in the conversion of naphthalene to salicylate
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
metabolism
NahF catalyzes the last reaction in the conversion of naphthalene to salicylate
Pseudomonas putida
Other publictions for EC 1.2.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743833
Jia
Evolutionary, computational, ...
Alteromonas naphthalenivorans
Sci. Rep.
7
43489
2017
-
-
1
-
4
-
1
9
-
-
1
1
-
4
-
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1
1
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2
2
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1
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8
1
1
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1
4
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1
1
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4
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1
4
9
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1
1
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1
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-
2
2
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1
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8
1
1
-
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1
5
5
1
8
8
741638
Singh
Purification and characteriza ...
Pseudomonas sp.
Appl. Biochem. Biotechnol.
172
806-819
2014
-
-
-
-
-
-
12
9
-
6
1
1
-
2
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1
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1
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26
1
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7
1
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2
7
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2
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12
7
9
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6
1
1
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1
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1
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26
1
-
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-
7
1
-
-
-
-
1
1
-
7
7
741793
Singh
Metabolic regulation and chro ...
Pseudomonas sp. C6
Arch. Microbiol.
195
521-535
2013
-
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1
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1
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2
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1
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1
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1
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1
1
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-
723872
Coitinho
Expression, purification and p ...
Pseudomonas putida, Pseudomonas putida G7
Acta Crystallogr. Sect. F
68
93-97
2012
-
-
1
1
-
-
-
-
-
-
1
-
-
11
-
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1
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2
1
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1
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1
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2
1
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1
1
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725888
Li
Physiological role of the nove ...
Pseudomonas putida, Pseudomonas putida ND6
Microbiol. Res.
166
643-653
2011
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7
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3
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4
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3
-
4
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1
1
1
1
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-
696505
Nayak
Metabolism of acenaphthylene v ...
Stenotrophomonas sp. RMSK
Biodegradation
20
837-843
2009
-
1
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2
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2
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2
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2
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1
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691830
Zhao
-
A novel salicylaldehyde dehydr ...
Pseudomonas putida, Pseudomonas putida ND6
Chin. Sci. Bull.
52
1942-1948
2007
-
1
1
-
-
-
8
4
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4
1
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4
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1
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28
1
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1
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1
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2
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2
2
2
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15
-
4
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5
2
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2
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28
2
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2
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2
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-
288315
Manohar
Degradation of naphthalene by ...
Pseudomonas sp., Pseudomonas sp. NGK1
Indian J. Exp. Biol.
33
353-356
1995
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2
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2
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1
1
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1
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1
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2
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1
1
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2
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288316
Eaton
Bacterial metabolism of naphth ...
Pseudomonas putida, Pseudomonas putida PpG1064
J. Bacteriol.
174
7542-7554
1992
-
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1
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2
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6
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1
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2
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1
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1
1
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2
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1
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2
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288317
Connors
Metabolism of naphthalene by p ...
Pseudomonas sp.
J. Bacteriol.
141
1052-1054
1980
-
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1
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1
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1
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1
2
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1
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1
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1
1
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1
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1
2
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1
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288318
Austen
-
Regulation of the plasmid-spec ...
Pseudomonas putida, Pseudomonas putida PpG7
J. Gen. Microbiol.
117
521-528
1980
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-
-
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2
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5
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1
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2
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1
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1
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2
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1
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2
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-
288319
Barnsley
Role and regulation of the ort ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Bacteriol.
125
404-408
1976
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3
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6
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2
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3
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2
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2
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3
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2
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3
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288320
Barnsley
The induction of the enzymes o ...
Pseudomonas putida, Pseudomonas putida PpG7, Pseudomonas sp.
J. Gen. Microbiol.
88
193-196
1975
-
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3
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6
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2
3
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3
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2
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2
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3
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2
3
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3
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288321
Shamsuzzaman
The regulation of naphthalene ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
60
582-589
1974
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1
1
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1
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1
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1
1
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1
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1
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1
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1
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