Expression, purification and preliminary crystallographic studies of NahF, a salicylaldehyde dehydrogenase from Pseudomonas putida G7 involved in naphthalene degradation

Coitinho, J.B.; Costa, D.M.; Guimaraes, S.L.; de Goes, A.M.; Nagem, R.A.; Acta Crystallogr. Sect. F 68, 93-97 (2012)

Data extracted from this reference:

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

additional information

nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase

723872

Pseudomonas putida

?

-

-

-

?

additional information

nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase

723872

Pseudomonas putida G7

?

-

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

additional information

nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase

723872

Pseudomonas putida

?

-

-

-

?

additional information

nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase

723872

Pseudomonas putida G7

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

743833

Jia

Evolutionary, computational, ...

Alteromonas naphthalenivorans

Sci. Rep.

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2017

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2

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Purification and characteriza ...

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723872

Coitinho

Expression, purification and p ...

Pseudomonas putida, Pseudomonas putida G7

Acta Crystallogr. Sect. F

68

93-97

2012

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Physiological role of the nove ...

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Metabolism of acenaphthylene v ...

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