BRENDA - Enzyme Database
show all sequences of 1.2.1.64

Redirection of metabolite biosynthesis from hydroxybenzoates to volatile terpenoids in green hairy roots of Daucus carota

Mukherjee, C.; Samanta, T.; Mitra, A.; Planta 243, 305-320 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
Daucus carota
-
4-hydroxybenzoate + NADH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Daucus carota
-
var. early nantes
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hairy root
green hairy root cultures. Hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots and 15fold less compared to normal hairy roots. Volatile analysis of 14-day-old green hairy roots reveals higher monoterpene and sesquiterpene contents than found in normal hairy roots, methyl salicylate content is higher in normal hairy roots than in green ones, overview
Daucus carota
-
root
-
Daucus carota
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000024
-
green hairy roots, pH 7.5, 35°C
Daucus carota
0.00036
-
normal hairy roots, pH 7.5, 35°C
Daucus carota
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
-
743556
Daucus carota
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Daucus carota
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Daucus carota
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Daucus carota
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Daucus carota
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
Daucus carota
-
4-hydroxybenzoate + NADH + 2 H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hairy root
green hairy root cultures. Hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots and 15fold less compared to normal hairy roots. Volatile analysis of 14-day-old green hairy roots reveals higher monoterpene and sesquiterpene contents than found in normal hairy roots, methyl salicylate content is higher in normal hairy roots than in green ones, overview
Daucus carota
-
root
-
Daucus carota
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000024
-
green hairy roots, pH 7.5, 35°C
Daucus carota
0.00036
-
normal hairy roots, pH 7.5, 35°C
Daucus carota
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
-
743556
Daucus carota
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Daucus carota
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Daucus carota
Expression
Organism
Commentary
Expression
Daucus carota
hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots
down
General Information
General Information
Commentary
Organism
malfunction
hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots. Volatile analysis of 14-day-old green hairy roots reveals higher monoterpene and sesquiterpene contents than found in normal hairy roots, methyl salicylate content is higher in normal hairy roots than in green ones, metabolic phenotype, overview
Daucus carota
metabolism
the enzyme is involved in the phenylpropanoid/hydroxybenzoate pathway in the plant, pathway overview
Daucus carota
physiological function
plant hydroxybenzoates are common mediators of plant responses of biotic and abiotic stress
Daucus carota
General Information (protein specific)
General Information
Commentary
Organism
malfunction
hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots. Volatile analysis of 14-day-old green hairy roots reveals higher monoterpene and sesquiterpene contents than found in normal hairy roots, methyl salicylate content is higher in normal hairy roots than in green ones, metabolic phenotype, overview
Daucus carota
metabolism
the enzyme is involved in the phenylpropanoid/hydroxybenzoate pathway in the plant, pathway overview
Daucus carota
physiological function
plant hydroxybenzoates are common mediators of plant responses of biotic and abiotic stress
Daucus carota
Expression (protein specific)
Organism
Commentary
Expression
Daucus carota
hairy roots cultures of Daucus carota turn green under continuous illumination, while the content of the major phenolic compound 4-hydroxybenzoic acid is reduced to half as compared to normal hairy roots cultured in darkness. 4-Hydroxybenzaldehyde dehydrogenase (HBD) activity is suppressed in the green hairy roots
down
Other publictions for EC 1.2.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743556
Mukherjee
Redirection of metabolite bio ...
Daucus carota
Planta
243
305-320
2016
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2
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2
2
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1
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1
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2
2
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1
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1
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1
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1
3
3
1
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723889
Sircar
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Characterization of p-hydroxyb ...
Daucus carota
Acta Physiol. Plant.
33
2019-2024
2011
2
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2
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7
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1
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2
1
11
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1
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1
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2
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2
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7
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2
1
11
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1
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1
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1
1
1
1
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688643
Sircar
Evidence for p-hydroxybenzoate ...
Daucus carota
J. Plant Physiol.
165
407-414
2008
1
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2
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1
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1
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1
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1
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1
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1
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1
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1
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1
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688652
Chakraborty
Accumulation of phenylpropanoi ...
Cocos nucifera
J. Plant Physiol.
166
63-71
2008
1
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2
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1
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1
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1
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1
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1
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1
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1
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288311
Yazaki
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Formation of p-hydroxybenzoic ...
Lithospermum erythrorhizon, Lithospermum erythrorhizon M18
Phytochemistry
30
2233-2236
1991
4
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1
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2
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2
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1
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10
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1
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4
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1
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1
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2
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1
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10
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288313
Bossert
Anaerobic oxidation of p-creso ...
Bacteria
J. Bacteriol.
171
2956-2962
1989
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1
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1
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1
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1
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1
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