BRENDA - Enzyme Database
show all sequences of 1.2.1.61

Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in Pseudomonas sp. 1-7

Zhang, S.; Sun, W.; Xu, L.; Zheng, X.; Chu, X.; Tian, J.; Wu, N.; Fan, Y.; BMC Microbiol. 12, 27 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
5 mM, 83.2% activity compared to untreated control
Pseudomonas sp.
SDS
5 mM, 81.6% activity compared to untreated control
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
5 mM chloride salt, 123.3% activity compared to untreated control
Pseudomonas sp.
Cd2+
5 mM sulfate salt, 58.9% activity compared to untreated control
Pseudomonas sp.
Co2+
5 mM chloride salt, 147.1% activity compared to untreated control
Pseudomonas sp.
Cu2+
5 mM chloride salt, 110.2% activity compared to untreated control
Pseudomonas sp.
Fe2+
5 mM chloride salt, 39.5% activity compared to untreated control
Pseudomonas sp.
Fe3+
5 mM chloride salt, 187.4% activity compared to untreated control
Pseudomonas sp.
K+
5 mM chloride salt, 95.7% activity compared to untreated control
Pseudomonas sp.
Mg2+
5 mM chloride salt, 138.1% activity compared to untreated control
Pseudomonas sp.
Mn2+
5 mM chloride salt, 186.1% activity compared to untreated control
Pseudomonas sp.
Na+
5 mM chloride salt, 88.2% activity compared to untreated control
Pseudomonas sp.
Ni2+
5 mM chloride salt, 183.9% activity compared to untreated control
Pseudomonas sp.
Zn2+
5 mM chloride salt, 145.9% activity compared to untreated control
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
recombinant His6-PdcG by SDS-PAGE
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
isolated from methyl parathion polluted activated sludge
-
Pseudomonas sp. 1-7
-
isolated from methyl parathion polluted activated sludge
-
Purification (Commentary)
Commentary
Organism
His6-PdcG by Ni2+-NTA affinity chromatography
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.44
-
purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
719179
Pseudomonas sp.
maleylacetate + NADH + 2 H+
-
-
-
?
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
719179
Pseudomonas sp. 1-7
maleylacetate + NADH + 2 H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Pseudomonas sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
70
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
purified enzyme retains 65% activity after 20 min at 60°C
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3
10
18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
5 mM, 83.2% activity compared to untreated control
Pseudomonas sp.
SDS
5 mM, 81.6% activity compared to untreated control
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
5 mM chloride salt, 123.3% activity compared to untreated control
Pseudomonas sp.
Cd2+
5 mM sulfate salt, 58.9% activity compared to untreated control
Pseudomonas sp.
Co2+
5 mM chloride salt, 147.1% activity compared to untreated control
Pseudomonas sp.
Cu2+
5 mM chloride salt, 110.2% activity compared to untreated control
Pseudomonas sp.
Fe2+
5 mM chloride salt, 39.5% activity compared to untreated control
Pseudomonas sp.
Fe3+
5 mM chloride salt, 187.4% activity compared to untreated control
Pseudomonas sp.
K+
5 mM chloride salt, 95.7% activity compared to untreated control
Pseudomonas sp.
Mg2+
5 mM chloride salt, 138.1% activity compared to untreated control
Pseudomonas sp.
Mn2+
5 mM chloride salt, 186.1% activity compared to untreated control
Pseudomonas sp.
Na+
5 mM chloride salt, 88.2% activity compared to untreated control
Pseudomonas sp.
Ni2+
5 mM chloride salt, 183.9% activity compared to untreated control
Pseudomonas sp.
Zn2+
5 mM chloride salt, 145.9% activity compared to untreated control
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
recombinant His6-PdcG by SDS-PAGE
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
His6-PdcG by Ni2+-NTA affinity chromatography
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.44
-
purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
719179
Pseudomonas sp.
maleylacetate + NADH + 2 H+
-
-
-
?
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
719179
Pseudomonas sp. 1-7
maleylacetate + NADH + 2 H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
70
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
purified enzyme retains 65% activity after 20 min at 60°C
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3
10
18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0
Pseudomonas sp.
General Information
General Information
Commentary
Organism
metabolism
enzyme is part of the degradation pathway of 4-nitrophenol which is a priority environmental pollutant
Pseudomonas sp.
General Information (protein specific)
General Information
Commentary
Organism
metabolism
enzyme is part of the degradation pathway of 4-nitrophenol which is a priority environmental pollutant
Pseudomonas sp.
Other publictions for EC 1.2.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742206
Su
Crystal structure of the gamm ...
Pseudomonas sp. WBC-3
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13
30
2013
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1
1
9
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1
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1
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1
1
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1
1
1
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1
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1
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1
1
1
9
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1
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1
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1
1
1
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1
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2
2
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719179
Zhang
Identification of the para-nit ...
Pseudomonas sp. 1-7, Pseudomonas sp.
BMC Microbiol.
12
27
2012
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1
-
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-
2
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12
1
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2
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1
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1
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2
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1
1
1
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1
1
1
1
-
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1
1
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2
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12
1
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1
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1
-
2
-
1
1
1
-
1
1
1
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-
1
1
-
-
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Kolvenbach
An unexpected gene cluster for ...
Sphingomonas sp., Sphingomonas sp. TTNP3
Appl. Microbiol. Biotechnol.
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1
-
-
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1
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4
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3
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2
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1
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1
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3
-
2
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-
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-
-
-
-
-
1
1
1
1
-
-
687422
Moonen
Elucidation of the 4-hydroxyac ...
Pseudomonas fluorescens, Pseudomonas fluorescens ACB
J. Bacteriol.
190
5190-5198
2008
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1
-
-
-
-
-
-
-
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1
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12
-
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2
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1
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1
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1
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2
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288304
Spain
Pathway for biodegradation of ...
Moraxella sp.
Appl. Environ. Microbiol.
57
812-819
1991
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1
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1
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1
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1
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1
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1
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