BRENDA - Enzyme Database
show all sequences of 1.2.1.60

Catabolism of 3- and 4-hydroxyphenylacetate by the 3,4-dihydroxyphenylacetate pathway in Escherichia coli

Cooper, R.A.; Skinner, M.A.; J. Bacteriol. 143, 302-306 (1980)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Escherichia coli
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Escherichia coli
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Escherichia coli B / ATCC 11303
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Escherichia coli B / ATCC 11303
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Escherichia coli C
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Escherichia coli C
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-
Escherichia coli C
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Escherichia coli
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288301
Escherichia coli
5-carboxymethyl-2-hydroxymuconate + NADH
-
288301
Escherichia coli
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288301
Escherichia coli B / ATCC 11303
5-carboxymethyl-2-hydroxymuconate + NADH
-
288301
Escherichia coli B / ATCC 11303
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288301
Escherichia coli C
5-carboxymethyl-2-hydroxymuconate + NADH
-
288301
Escherichia coli C
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
1,5% of the activity compared to reduction with NAD+
288301
Escherichia coli
5-carboxymethyl-2-hydroxymuconate + NADPH
-
288301
Escherichia coli
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
1,5% of the activity compared to reduction with NAD+
288301
Escherichia coli B / ATCC 11303
5-carboxymethyl-2-hydroxymuconate + NADPH
-
288301
Escherichia coli B / ATCC 11303
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
1,5% of the activity compared to reduction with NAD+
288301
Escherichia coli C
5-carboxymethyl-2-hydroxymuconate + NADPH
-
288301
Escherichia coli C
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADP+
98.5% lower activity compared to NAD+
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADP+
98.5% lower activity compared to NAD+
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Escherichia coli
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Escherichia coli
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Escherichia coli B / ATCC 11303
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Escherichia coli B / ATCC 11303
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
Escherichia coli C
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
5-carboxymethyl-2-hydroxymuconate + NADH
-
Escherichia coli C
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Escherichia coli
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288301
Escherichia coli
5-carboxymethyl-2-hydroxymuconate + NADH
-
288301
Escherichia coli
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288301
Escherichia coli B / ATCC 11303
5-carboxymethyl-2-hydroxymuconate + NADH
-
288301
Escherichia coli B / ATCC 11303
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
involved in the meta-cleavage pathway of 4-hydroxyphenylacetate
288301
Escherichia coli C
5-carboxymethyl-2-hydroxymuconate + NADH
-
288301
Escherichia coli C
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
1,5% of the activity compared to reduction with NAD+
288301
Escherichia coli
5-carboxymethyl-2-hydroxymuconate + NADPH
-
288301
Escherichia coli
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
1,5% of the activity compared to reduction with NAD+
288301
Escherichia coli B / ATCC 11303
5-carboxymethyl-2-hydroxymuconate + NADPH
-
288301
Escherichia coli B / ATCC 11303
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
1,5% of the activity compared to reduction with NAD+
288301
Escherichia coli C
5-carboxymethyl-2-hydroxymuconate + NADPH
-
288301
Escherichia coli C
?
Other publictions for EC 1.2.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288297
Fawcett
5-Carboxymethyl-2-hydroxymucon ...
Escherichia coli, Escherichia coli C, Klebsiella pneumoniae, Klebsiella pneumoniae M5a1
FEMS Microbiol. Lett.
57
307-312
1989
-
-
-
-
-
-
-
4
-
-
4
4
-
19
-
-
2
-
-
2
3
-
4
2
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
4
4
-
-
-
2
-
2
3
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
288298
Alonso
-
Carboxymethylhydroxymuconic se ...
Pseudomonas putida
Biochem. Cell Biol.
64
1288-1293
1986
-
-
-
-
-
-
1
4
-
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
2
1
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
2
1
-
-
-
1
-
1
1
1
2
1
-
-
2
1
1
-
-
-
-
-
-
-
-
-
288299
Alonso
Kinetic properties of 5-carbox ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochimie
68
731-737
1986
-
-
-
-
-
-
2
2
-
-
-
2
-
178
-
-
1
-
-
1
-
-
2
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
2
-
-
-
1
-
1
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
288300
Alonso
Carboxymethylhydroxymuconic se ...
Escherichia coli
Biochim. Biophys. Acta
719
165-167
1982
-
-
-
-
-
-
3
3
-
-
2
1
-
2
-
-
1
-
-
1
1
-
2
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
1
-
-
-
1
-
1
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288303
Barbour
Control of meta-cleavage degra ...
Pseudomonas putida, Pseudomonas putida NCIB 9865
J. Bacteriol.
147
844-850
1981
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288301
Cooper
Catabolism of 3- and 4-hydroxy ...
Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia coli C
J. Bacteriol.
143
302-306
1980
-
-
-
-
-
-
-
-
-
-
-
3
-
184
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288040
Blakley
The catabolism of L-tyrosine b ...
Arthrobacter globiformis, Arthrobacter globiformis PRL W15
Can. J. Microbiol.
23
1128-1139
1977
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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-
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