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Literature summary for 1.2.1.57 extracted from

  • Bhandiwad, A.; Shaw, A.; Guss, A.; Guseva, A.; Bahl, H.; Lynd, L.
    Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production (2014), Metab. Eng., 21, 17-25 .
    View publication on PubMed
Search for more literature for 1.2.1.57

Cloned(Commentary)

Cloned (Comment) Organism
gene bcd, the genes (crt, bcd, etfB, etfA, hbd, and thl) responsible for butyryl-CoA formation occur as a multi-gene operon, plasmid construction map for expressing individual genes of the n-butanol pathway, plasmids pHBD,pCRT,pBCD-etfAB, and pADHE, overview Thermoanaerobacterium saccharolyticum

Protein Variants

Protein Variants Comment Organism
additional information recombinant expression of the enzyme from Clostridium acetobutylicum strain ATCC 824 in Thermoanaerobacterium saccharolyticum strain DSM 8691 Thermoanaerobacterium saccharolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butanoyl-CoA + NADH + H+ Thermoanaerobacterium saccharolyticum
-
 butanal + CoA + NAD+
-
 ?
butanoyl-CoA + NADH + H+ Thermoanaerobacterium saccharolyticum DSM 571
-
 butanal + CoA + NAD+
-
 ?
butanoyl-CoA + NADPH + H+ Thermoanaerobacterium saccharolyticum
-
 butanal + CoA + NADP+
-
 ?
butanoyl-CoA + NADPH + H+ Thermoanaerobacterium saccharolyticum DSM 571
-
 butanal + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum
-
 formerly Clostridium thermosaccharolyticum
-
Thermoanaerobacterium saccharolyticum DSM 571
-
 formerly Clostridium thermosaccharolyticum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.67
-
 pH 7.0, 55°C, substrate butanoyl-CoA Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanoyl-CoA + FADH2
-
 Thermoanaerobacterium saccharolyticum butanal + CoA + FAD
-
 ?
butanoyl-CoA + FADH2
-
 Thermoanaerobacterium saccharolyticum DSM 571 butanal + CoA + FAD
-
 ?
butanoyl-CoA + NADH + H+
-
 Thermoanaerobacterium saccharolyticum butanal + CoA + NAD+
-
 ?
butanoyl-CoA + NADH + H+
-
 Thermoanaerobacterium saccharolyticum DSM 571 butanal + CoA + NAD+
-
 ?
butanoyl-CoA + NADPH + H+
-
 Thermoanaerobacterium saccharolyticum butanal + CoA + NADP+
-
 ?
butanoyl-CoA + NADPH + H+
-
 Thermoanaerobacterium saccharolyticum DSM 571 butanal + CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
BCD
-
 Thermoanaerobacterium saccharolyticum
butyryl CoA dehydrogenase
-
 Thermoanaerobacterium saccharolyticum
Tthe_1660
-
 Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Thermoanaerobacterium saccharolyticum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Thermoanaerobacterium saccharolyticum
NADPH
-
 Thermoanaerobacterium saccharolyticum