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Literature summary for 1.2.1.57 extracted from

  • Hwang, H.J.; Park, J.H.; Kim, J.H.; Kong, M.K.; Kim, J.W.; Park, J.W.; Cho, K.M.; Lee, P.C.
    Engineering of a butyraldehyde dehydrogenase of Clostridium saccharoperbutylacetonicum to fit an engineered 1,4-butanediol pathway in Escherichia coli (2014), Biotechnol. Bioeng., 111, 1374-1384 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bld, butyraldehyde dehydrogenase gene of Clostridium saccharoperbutylacetonicum is coexpresses with diverse involved genes in Escherichia coli for the reconstruction of 1,4-butanediol biosynthesis pathway in Escherichia coli, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Clostridium saccharoperbutylacetonicum

Protein Variants

Protein Variants Comment Organism
A176T random mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
A467S random mutagenesis, the mutant shows similar activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
K279R random mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
L273C site-directed mutagenesis, the mutant shows about 2fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
L273I random mutagenesis, the mutant shows about 3.5fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
L273M site-directed mutagenesis, the mutant shows about 2fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
L273S site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
L273T site-directed mutagenesis, the mutant shows about 4fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
L273V site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
M371R random mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
additional information to fit enzyme Bld into the non-natural 1,4-butanediol pathway, it is engineered through random mutagenesis, library construction. Five Bld mutants are isolated using a colorimetric Schiff's reagent-based method. Subsequent site-directed mutagenesis of Bld generates the two best Bld mutants, L273I and L273T, which produce 1,4-butanediol titers fourfold greater than those of wild-type Bld. Butyraldehyde dehydrogenase (Bld) and butanol dehydrogenase (Bdh) of Clostridium saccharoperbutylacetonicum are selected as a substitute for the bifunctional AdhE2 in the reconstructed 1,4-butanediol biosynthesis pathway in Escherichia coli, coexpression of diverse involved genes in Escherichia coli Clostridium saccharoperbutylacetonicum
N409T random mutagenesis, the mutant shows similar activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information no Michaelis-Menten behaviour Clostridium saccharoperbutylacetonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybutanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum
-
4-hydroxybutanal + CoA + NAD+
-
?
4-hydroxybutanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
4-hydroxybutanal + CoA + NAD+
-
?
4-hydroxybutanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum
-
4-hydroxybutanal + CoA + NADP+
-
?
4-hydroxybutanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
4-hydroxybutanal + CoA + NADP+
-
?
butanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum
-
butanal + CoA + NAD+
-
?
butanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
butanal + CoA + NAD+
-
?
butanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum
-
butanal + CoA + NADP+
-
?
butanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
butanal + CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Clostridium saccharoperbutylacetonicum
-
-
-
Clostridium saccharoperbutylacetonicum KCTC5577
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybutanoyl-CoA + NADH + H+
-
Clostridium saccharoperbutylacetonicum 4-hydroxybutanal + CoA + NAD+
-
?
4-hydroxybutanoyl-CoA + NADH + H+
-
Clostridium saccharoperbutylacetonicum KCTC5577 4-hydroxybutanal + CoA + NAD+
-
?
4-hydroxybutanoyl-CoA + NADPH + H+
-
Clostridium saccharoperbutylacetonicum 4-hydroxybutanal + CoA + NADP+
-
?
4-hydroxybutanoyl-CoA + NADPH + H+
-
Clostridium saccharoperbutylacetonicum KCTC5577 4-hydroxybutanal + CoA + NADP+
-
?
butanoyl-CoA + NADH + H+
-
Clostridium saccharoperbutylacetonicum butanal + CoA + NAD+
-
?
butanoyl-CoA + NADH + H+
-
Clostridium saccharoperbutylacetonicum KCTC5577 butanal + CoA + NAD+
-
?
butanoyl-CoA + NADPH + H+
-
Clostridium saccharoperbutylacetonicum butanal + CoA + NADP+
-
?
butanoyl-CoA + NADPH + H+
-
Clostridium saccharoperbutylacetonicum KCTC5577 butanal + CoA + NADP+
-
?

Synonyms

Synonyms Comment Organism
Bld
-
Clostridium saccharoperbutylacetonicum
Butyraldehyde dehydrogenase
-
Clostridium saccharoperbutylacetonicum

Cofactor

Cofactor Comment Organism Structure
NADH
-
Clostridium saccharoperbutylacetonicum
NADPH
-
Clostridium saccharoperbutylacetonicum

General Information

General Information Comment Organism
metabolism the enzyme is part of the 1,4-butanediol biosynthesis pathway, overview Clostridium saccharoperbutylacetonicum