BRENDA - Enzyme Database show
show all sequences of 1.2.1.51

Purification and characterization of pyruvate:NADP+ oxidoreductase in Euglena gracilis

Inui, H.; Ono, K.; Miyatake, K.; Nakano, Y.; Kitaoka, S.; J. Biol. Chem. 262, 9130-9135 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not arsenite
Euglena gracilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
CoA
-
Euglena gracilis
0.03
-
NADP+
-
Euglena gracilis
0.21
-
pyruvate
-
Euglena gracilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
4 mol per mol subunit
Euglena gracilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
166000
-
2 * 166000, SDS-PAGE
Euglena gracilis
309000
-
gel filtration; I. methyl viologen-pyruvate dehydrogenase fragment with iron-sulfur cluster; two covalently linked domains
Euglena gracilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + CoA + NADP+
Euglena gracilis
-
acetyl-CoA + CO2 + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Euglena gracilis
-
-
-
Oxidation Stability
Oxidation Stability
Organism
O2 leads to rapid inactivation
Euglena gracilis
Purification (Commentary)
Commentary
Organism
-
Euglena gracilis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.93
-
3-hydroxypyruvate
Euglena gracilis
2.36
-
2-oxobutyrate
Euglena gracilis
2.6
-
oxaloacetate
Euglena gracilis
14.3
-
pyruvate
Euglena gracilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + CoA + NADP+
-
288281
Euglena gracilis
propionyl-CoA + CO2 + NADPH
-
-
-
?
3-hydroxypyruvate + CoA + NADP+
-
288281
Euglena gracilis
hydoxyacetyl-CoA + CO2 + NADPH
-
-
-
?
oxaloacetate + CoA + NADP+
-
288281
Euglena gracilis
? + CO2 + NADPH
-
-
-
?
pyruvate + CoA + NADP+
-
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
pyruvate + CoA + NADP+
NADP+ can be substituted by methyl viologen
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
pyruvate + CoA + NADP+
NADP+ can be substituted by benzyl viologen
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
pyruvate + CoA + NADP+
reverse reaction 2% of forward reaction
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 166000, SDS-PAGE
Euglena gracilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Euglena gracilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Euglena gracilis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Euglena gracilis
NADP+
not NAD+
Euglena gracilis
thiamine diphosphate
-
Euglena gracilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Euglena gracilis
NADP+
not NAD+
Euglena gracilis
thiamine diphosphate
-
Euglena gracilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not arsenite
Euglena gracilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
CoA
-
Euglena gracilis
0.03
-
NADP+
-
Euglena gracilis
0.21
-
pyruvate
-
Euglena gracilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
4 mol per mol subunit
Euglena gracilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
166000
-
2 * 166000, SDS-PAGE
Euglena gracilis
309000
-
gel filtration; I. methyl viologen-pyruvate dehydrogenase fragment with iron-sulfur cluster; two covalently linked domains
Euglena gracilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + CoA + NADP+
Euglena gracilis
-
acetyl-CoA + CO2 + NADPH
-
-
r
Oxidation Stability (protein specific)
Oxidation Stability
Organism
O2 leads to rapid inactivation
Euglena gracilis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Euglena gracilis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.93
-
3-hydroxypyruvate
Euglena gracilis
2.36
-
2-oxobutyrate
Euglena gracilis
2.6
-
oxaloacetate
Euglena gracilis
14.3
-
pyruvate
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + CoA + NADP+
-
288281
Euglena gracilis
propionyl-CoA + CO2 + NADPH
-
-
-
?
3-hydroxypyruvate + CoA + NADP+
-
288281
Euglena gracilis
hydoxyacetyl-CoA + CO2 + NADPH
-
-
-
?
oxaloacetate + CoA + NADP+
-
288281
Euglena gracilis
? + CO2 + NADPH
-
-
-
?
pyruvate + CoA + NADP+
-
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
pyruvate + CoA + NADP+
NADP+ can be substituted by methyl viologen
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
pyruvate + CoA + NADP+
NADP+ can be substituted by benzyl viologen
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
pyruvate + CoA + NADP+
reverse reaction 2% of forward reaction
288281
Euglena gracilis
acetyl-CoA + CO2 + NADPH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 166000, SDS-PAGE
Euglena gracilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Euglena gracilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Euglena gracilis
Other publictions for EC 1.2.1.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704746
Sawada
Key role for transketolase act ...
Moniliella megachiliensis, Moniliella megachiliensis SN-G42
J. Biosci. Bioeng.
108
385-390
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
675116
Ctrnacta
Localization of pyruvate:NADP+ ...
Cryptosporidium parvum
J. Eukaryot. Microbiol.
53
225-231
2006
-
-
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654355
Nakazawa
Pyruvate:NADP+ oxidoreductase ...
Euglena gracilis
Arch. Biochem. Biophys.
411
183-188
2003
1
-
-
-
-
1
-
-
2
-
2
1
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288284
Nakazawa
The origin of pyruvate: NADP o ...
Euglena gracilis
FEBS Lett.
479
155-156
2000
-
-
1
-
-
-
-
-
1
1
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288278
Inui
Pyruvate:NADP+ oxidoreductase ...
Euglena gracilis
Arch. Biochem. Biophys.
286
270-276
1991
-
-
-
-
-
-
-
-
1
1
1
1
-
2
1
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
1
1
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288279
Inui
Pyruvate: NADP+ oxidoreductase ...
Euglena gracilis
Arch. Biochem. Biophys.
280
292-298
1990
-
-
-
-
-
-
-
-
1
-
-
1
-
4
1
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288285
Lochmeyer
-
NADP+-specific 2-oxoglutarate ...
Euglena gracilis
Arch. Microbiol.
153
226-229
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
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-
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-
-
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-
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-
-
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-
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-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288280
Inui
Pyruvate: NADP+ oxidoreductase ...
Euglena gracilis
Arch. Biochem. Biophys.
274
434-442
1989
-
-
-
-
-
-
4
7
-
-
-
1
-
2
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
7
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288281
Inui
Purification and characterizat ...
Euglena gracilis
J. Biol. Chem.
262
9130-9135
1987
-
-
-
-
-
-
1
3
-
1
2
1
-
5
1
-
1
-
-
1
4
-
7
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
3
-
1
2
1
-
1
-
1
-
1
4
-
7
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288282
Inui
The physiological role of oxyg ...
Euglena gracilis
Arch. Biochem. Biophys.
237
423-429
1985
-
-
-
-
-
-
-
-
2
-
-
1
-
3
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288283
Inui
Occurrence of oxygen-sensitive ...
Euglena gracilis
J. Biochem.
96
931-934
1984
-
-
-
-
-
-
-
-
3
-
-
1
-
3
1
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
3
-
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-
-
-
-
3
-
-
-
-
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-
-
3
-
-
1
-
1
-
1
-
2
-
-
1
-
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