BRENDA - Enzyme Database show
show all sequences of 1.2.1.5

Alternative biotransformation of retinal to retinoic acid or retinol by an aldehyde dehydrogenase from Bacillus cereus

Hong, S.H.; Ngo, H.P.; Nam, H.K.; Kim, K.R.; Kang, L.W.; Oh, D.K.; Appl. Environ. Microbiol. 82, 3940-3946 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Bacillus cereus
Engineering
Amino acid exchange
Commentary
Organism
C300A
the variant shows no oxidation activity
Bacillus cereus
E194S
the variant shows 15fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme
Bacillus cereus
E266A
the variant shows no oxidation activity
Bacillus cereus
E457V
the variant shows 7.5fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme
Bacillus cereus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0037
-
NAD+
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.004
-
NAD+
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.00625
-
NAD+
mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.007
-
all-trans-retinal
with NAD+ as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.01
-
NAD+
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.095
-
acetaldehyde
at pH 7.0 and 37°C
Bacillus cereus
0.2
-
benzaldehyde
at pH 7.0 and 37°C
Bacillus cereus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
4 * 54000, SDS-PAGE
Bacillus cereus
216000
-
gel filtration
Bacillus cereus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
C2N217
-
-
Bacillus cereus ATCC 10876
C2N217
-
-
Purification (Commentary)
Commentary
Organism
Sephacryl S-300 gel filtration
Bacillus cereus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
-
739913
Bacillus cereus
acetate + NADH + H+
-
-
-
?
acetaldehyde + NAD+ + H2O
-
739913
Bacillus cereus ATCC 10876
acetate + NADH + H+
-
-
-
?
all-trans-retinal + NAD+ + H2O
-
739913
Bacillus cereus
all-trans-retinoic acid + NADH + H+
-
-
-
?
all-trans-retinal + NAD+ + H2O
-
739913
Bacillus cereus ATCC 10876
all-trans-retinoic acid + NADH + H+
-
-
-
?
all-trans-retinal + NADP+ + H2O
-
739913
Bacillus cereus
all-trans-retinol + NADPH + H+
-
-
-
?
all-trans-retinal + NADP+ + H2O
-
739913
Bacillus cereus ATCC 10876
all-trans-retinol + NADPH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
739913
Bacillus cereus
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
739913
Bacillus cereus ATCC 10876
benzoate + NADH + H+
-
-
-
?
additional information
no activity with 9-cis-retinal and 13-cis-retinal
739913
Bacillus cereus
?
-
-
-
-
additional information
no activity with 9-cis-retinal and 13-cis-retinal
739913
Bacillus cereus ATCC 10876
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 54000, SDS-PAGE
Bacillus cereus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0007
-
NAD+
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.0017
-
NAD+
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.005
-
all-trans-retinal
with NAD+ as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.005
-
NAD+
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.026
-
NAD+
mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.183
-
acetaldehyde
at pH 7.0 and 37°C
Bacillus cereus
0.217
-
benzaldehyde
at pH 7.0 and 37°C
Bacillus cereus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus cereus
NADP+
-
Bacillus cereus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Bacillus cereus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus cereus
NADP+
-
Bacillus cereus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C300A
the variant shows no oxidation activity
Bacillus cereus
E194S
the variant shows 15fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme
Bacillus cereus
E266A
the variant shows no oxidation activity
Bacillus cereus
E457V
the variant shows 7.5fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme
Bacillus cereus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0037
-
NAD+
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.004
-
NAD+
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.00625
-
NAD+
mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.007
-
all-trans-retinal
with NAD+ as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.01
-
NAD+
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.095
-
acetaldehyde
at pH 7.0 and 37°C
Bacillus cereus
0.2
-
benzaldehyde
at pH 7.0 and 37°C
Bacillus cereus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
4 * 54000, SDS-PAGE
Bacillus cereus
216000
-
gel filtration
Bacillus cereus
Purification (Commentary) (protein specific)
Commentary
Organism
Sephacryl S-300 gel filtration
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
-
739913
Bacillus cereus
acetate + NADH + H+
-
-
-
?
acetaldehyde + NAD+ + H2O
-
739913
Bacillus cereus ATCC 10876
acetate + NADH + H+
-
-
-
?
all-trans-retinal + NAD+ + H2O
-
739913
Bacillus cereus
all-trans-retinoic acid + NADH + H+
-
-
-
?
all-trans-retinal + NAD+ + H2O
-
739913
Bacillus cereus ATCC 10876
all-trans-retinoic acid + NADH + H+
-
-
-
?
all-trans-retinal + NADP+ + H2O
-
739913
Bacillus cereus
all-trans-retinol + NADPH + H+
-
-
-
?
all-trans-retinal + NADP+ + H2O
-
739913
Bacillus cereus ATCC 10876
all-trans-retinol + NADPH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
739913
Bacillus cereus
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
739913
Bacillus cereus ATCC 10876
benzoate + NADH + H+
-
-
-
?
additional information
no activity with 9-cis-retinal and 13-cis-retinal
739913
Bacillus cereus
?
-
-
-
-
additional information
no activity with 9-cis-retinal and 13-cis-retinal
739913
Bacillus cereus ATCC 10876
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 54000, SDS-PAGE
Bacillus cereus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0007
-
NAD+
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.0017
-
NAD+
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.005
-
all-trans-retinal
with NAD+ as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.005
-
NAD+
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.026
-
NAD+
mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.183
-
acetaldehyde
at pH 7.0 and 37°C
Bacillus cereus
0.217
-
benzaldehyde
at pH 7.0 and 37°C
Bacillus cereus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.42
-
NAD+
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.5
-
NAD+
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.67
-
all-trans-retinal
with NAD+ as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
1.08
-
benzaldehyde
at pH 7.0 and 37°C
Bacillus cereus
1.85
-
acetaldehyde
at pH 7.0 and 37°C
Bacillus cereus
4.1
-
NAD+
mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.42
-
NAD+
wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.5
-
NAD+
mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
0.67
-
all-trans-retinal
with NAD+ as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
1.08
-
benzaldehyde
at pH 7.0 and 37°C
Bacillus cereus
1.85
-
acetaldehyde
at pH 7.0 and 37°C
Bacillus cereus
4.1
-
NAD+
mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C
Bacillus cereus
Other publictions for EC 1.2.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735898
Alam
Human salivary aldehyde dehydr ...
Homo sapiens
Cell Biochem. Biophys.
74
307-315
2016
3
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
1
-
-
5
-
1
-
2
-
1
-
-
1
-
-
-
3
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
1
-
-
5
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
739913
Hong
Alternative biotransformation ...
Bacillus cereus, Bacillus cereus ATCC 10876
Appl. Environ. Microbiol.
82
3940-3946
2016
-
-
1
-
4
-
-
7
-
-
2
-
-
3
-
-
1
-
-
-
-
-
10
1
-
-
-
7
-
-
-
2
-
-
-
-
-
1
2
-
4
-
-
-
-
7
-
-
2
-
-
-
-
1
-
-
-
-
10
1
-
-
-
7
-
-
-
-
-
-
-
-
7
7
740279
Mali
Impaired ALDH2 activity decrea ...
Rattus norvegicus
Cell. Signal.
28
1-6
2016
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741087
Tomita
Aldehyde dehydrogenase 1A1 in ...
Homo sapiens
Oncotarget
7
11018-11032
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
741167
Jimenez-Lopez
-
Narrow-leafed lupin (Lupinus a ...
Lupinus angustifolius
Plant Gene
6
67-76
2016
-
-
-
-
-
-
-
-
5
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740294
Gonzalez-Segura
Residues that influence coenzy ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
Chem. Biol. Interact.
234
59-74
2015
-
-
-
-
-
-
-
2
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
722210
Liu
Molecular characterization of ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Extremophiles
17
181-190
2013
1
-
1
-
-
1
11
5
-
4
3
-
11
3
-
-
1
-
-
-
-
-
17
1
1
1
3
5
1
1
-
2
-
-
-
1
-
1
2
-
-
1
-
11
-
5
-
4
3
-
11
-
-
1
-
-
-
-
17
1
1
1
3
5
1
1
-
-
-
-
-
-
5
5
723877
Ngo
Expression, crystallization an ...
Bacillus cereus, Bacillus cereus ATCC 10876
Acta Crystallogr. Sect. F
69
528-531
2013
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724620
Zhou
Production of 3-hydroxypropion ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Biotechnol. Bioeng.
110
3177-3187
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724720
Gonzalez-Segura
Catalytic contribution of thre ...
Homo sapiens
Chem. Biol. Interact.
202
32-40
2013
-
-
1
1
4
-
-
10
1
1
1
2
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
10
-
-
-
1
-
-
-
-
-
1
1
1
4
-
-
-
-
10
1
1
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
10
-
-
-
-
-
-
-
-
10
10
725085
Zhou
Aldehyde dehydrogenase protein ...
Zea mays
Funct. Integr. Genomics
12
683-691
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
747332
Aquino Neto
Development of nanostructured ...
Saccharomyces cerevisiae
Biosens. Bioelectron.
26
2922-2926
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
711110
Koenig
Aldehyde dehydrogenase 1A3 is ...
Homo sapiens
Biochem. Biophys. Res. Commun.
400
207-211
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
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1
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1
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
711635
Stagos
Corneal aldehyde dehydrogenase ...
Mus musculus
Brain Res. Bull.
81
211-218
2010
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
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2
-
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-
-
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-
-
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-
1
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2
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725948
Lo
Gene cloning and biochemical c ...
Bacillus licheniformis, Bacillus licheniformis ATCC 14580
Mol. Biotechnol.
46
157-167
2010
2
-
1
-
-
-
15
10
-
1
3
-
-
3
-
-
1
-
-
-
-
-
17
1
1
-
1
10
1
-
1
2
-
-
-
2
-
1
2
-
-
-
-
15
-
10
-
1
3
-
-
-
-
1
-
-
-
-
17
1
1
-
1
10
1
-
1
-
-
-
-
-
10
10
698095
Gao
Evolutionary and expression st ...
Oryza sativa
Gene
431
86-94
2009
-
-
1
-
-
-
-
-
2
-
11
9
-
9
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
9
18
-
-
-
-
-
-
-
5
-
13
9
-
-
-
-
-
9
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684586
Maestre
Effects of ADH2 overexpression ...
Lactobacillus casei BL23
Appl. Environ. Microbiol.
74
702-707
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
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-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684721
Glatt
Detoxification of promutagenic ...
Homo sapiens
Arch. Biochem. Biophys.
477
196-205
2008
-
-
1
-
-
-
-
8
1
-
-
-
-
1
-
-
1
-
-
3
-
-
9
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
8
1
-
-
-
-
-
-
1
-
3
-
-
9
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
685040
Ho
A point mutation produced a cl ...
Homo sapiens
Biochem. Pharmacol.
76
690-696
2008
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686531
Quash
Aldehyde dehydrogenase inhibit ...
Homo sapiens
Eur. J. Med. Chem.
43
906-916
2008
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688414
Bajns J.
Structural and biochemical cha ...
Paraburkholderia xenovorans, Paraburkholderia xenovorans LB400
J. Mol. Biol.
379
597-608
2008
-
-
1
1
-
-
-
8
-
-
1
-
-
2
-
-
1
-
-
-
-
-
8
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
8
-
-
1
-
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690142
Alnouti
Tissue distribution, ontogeny, ...
Mus musculus
Toxicol. Sci.
101
51-64
2008
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
15
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
45
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692251
Marchitti
Non-P450 aldehyde oxidizing en ...
Homo sapiens
Expert. Opin. Drug Metab. Toxicol.
4
697-720
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688446
Szoecs
Increased superoxide productio ...
Rattus norvegicus, Saccharomyces cerevisiae
J. Mol. Cell. Cardiol.
42
1111-1118
2007
-
2
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689379
Marchitti
Neurotoxicity and metabolism o ...
Homo sapiens
Pharmacol. Rev.
59
125-150
2007
-
-
-
-
-
-
1
5
3
-
-
-
-
3
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
3
-
6
5
-
-
-
-
-
-
-
-
6
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671511
Ohta
A novel nicotinoprotein aldehy ...
Sphingopyxis macrogoltabida 103, Sphingopyxis macrogoltabida
Appl. Microbiol. Biotechnol.
68
639-646
2005
-
1
1
-
-
-
8
1
-
1
2
2
-
3
-
-
1
-
-
1
2
-
24
1
1
-
-
-
1
-
-
2
-
-
-
-
1
1
2
-
-
-
-
8
-
1
-
1
2
2
-
-
-
1
-
1
2
-
24
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656571
Oikawa
-
Paradoxical thermostable enzym ...
Cytophaga sp.
J. Mol. Catal. B
23
65-70
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
22
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
22
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
654425
Yamanaka
Thermostable aldehyde dehydrog ...
Cytophaga sp.
Biochem. Biophys. Res. Commun.
298
632-637
2002
-
-
1
-
-
-
-
3
-
-
2
-
-
3
-
-
1
-
-
-
1
1
24
1
1
-
2
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
1
24
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
348696
Izaguirre
Human aldehyde dehydrogenase c ...
Homo sapiens
J. Biomol. Struct. Dyn.
19
429-447
2001
-
-
-
-
-
-
2
13
2
-
-
-
-
3
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
13
2
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348798
Schraeder
NAD(P)-dependent aldehyde dehy ...
Cupriavidus necator, Cupriavidus necator Bo
J. Bacteriol.
183
7408-7411
2001
-
-
-
-
-
-
2
1
-
-
3
2
-
2
-
-
1
-
-
-
-
1
12
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
1
-
-
3
2
-
-
-
1
-
-
-
1
12
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
655423
Boubekeur
Participation of acetaldehyde ...
Saccharomyces cerevisiae
Eur. J. Biochem.
268
5057-5065
2001
-
-
-
-
-
-
-
-
1
1
-
1
-
2
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348797
Perozich
Shifting the NAD/NADP preferen ...
Rattus norvegicus
Eur. J. Biochem.
267
6197-6203
2000
-
-
-
-
6
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348783
Wang
Molecular cloning, characteriz ...
Saccharomyces cerevisiae
J. Bacteriol.
180
822-830
1998
-
-
1
-
-
-
1
18
2
4
2
-
-
1
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
18
2
4
2
-
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348780
Downes
Purification and properties of ...
Mus musculus
Biochem. Mol. Biol. Int.
30
525-535
1993
-
-
-
-
-
-
1
7
-
-
2
1
-
1
-
-
1
-
-
1
1
-
8
1
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
-
2
1
-
-
-
1
-
1
1
-
8
1
-
-
-
7
-
-
-
-
-
-
-
-
-
-
348782
King
Human corneal aldehyde dehydro ...
Homo sapiens
Biochem. Mol. Biol. Int.
31
49-63
1993
-
-
-
-
-
-
1
6
-
-
-
1
-
3
-
-
1
-
-
1
1
-
7
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
1
-
-
-
1
-
1
1
-
7
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
348784
Sreerama
Identification and characteriz ...
Homo sapiens
Biochem. Pharmacol.
45
2487-2505
1993
-
-
-
-
-
1
-
17
-
-
5
-
-
2
-
-
1
-
-
3
2
1
7
1
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
17
-
-
5
-
-
-
-
1
-
3
2
1
7
1
1
-
-
-
2
-
2
-
-
-
-
-
-
-
348781
Choi
-
Purification and characterizat ...
Bos taurus
Korean Biochem. J.
24
224-230
1991
-
-
-
-
-
-
-
8
1
-
2
1
-
1
-
1
1
-
-
1
1
-
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
1
-
2
1
-
-
1
1
-
1
1
-
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
348788
Jeng
Purification and characterizat ...
Rattus norvegicus
Arch. Biochem. Biophys.
289
214-222
1991
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
348787
Evces
Characterization of rat cornea ...
Rattus norvegicus
Arch. Biochem. Biophys.
274
518-524
1989
-
-
-
-
-
-
1
10
-
-
2
1
-
2
-
-
1
-
-
3
1
-
16
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
10
-
-
2
1
-
-
-
1
-
3
1
-
16
1
-
-
1
1
2
-
-
-
-
-
-
-
-
-
348786
Senior
Purification and characterizat ...
Rattus norvegicus
Arch. Biochem. Biophys.
262
211-220
1988
-
-
-
-
-
-
2
-
1
-
2
-
-
1
-
-
1
-
-
1
2
1
12
1
-
-
1
-
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
2
4
-
1
-
2
-
-
-
-
1
-
1
2
1
12
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
348789
Rietveld
Substituent effects during the ...
Rattus norvegicus, Saccharomyces cerevisiae
Biochim. Biophys. Acta
914
162-169
1987
-
-
-
-
-
-
-
27
2
-
-
-
-
4
-
-
-
-
-
3
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
27
2
-
-
-
-
-
-
-
-
3
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348790
Lindahl
Characterization of aldehyde d ...
Rattus norvegicus
Biochim. Biophys. Acta
843
180-185
1985
-
-
-
-
-
-
1
4
-
-
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
1
-
-
-
-
1
-
1
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
348792
Eckfeldt
Isozymes of aldehyde dehydroge ...
Equus caballus
Methods Enzymol.
89
474-479
1982
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4
17
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4
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1
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1
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1
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10
1
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2
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2
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4
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17
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4
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1
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1
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10
1
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348793
Tamaki
Aldehyde dehydrogenase from ba ...
Saccharomyces cerevisiae
Methods Enzymol.
89
469-473
1982
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1
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11
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2
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1
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1
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1
1
11
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1
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348795
Bostian
Kinetics and reaction mechanis ...
Saccharomyces cerevisiae
Biochem. J.
173
787-798
1978
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2
1
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1
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1
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1
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3
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1
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348794
Tamaki
Purification and properties of ...
Saccharomyces cerevisiae
J. Biochem.
82
73-79
1977
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2
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1
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13
1
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1
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1
1
13
1
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1
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348796
Eckfeldt
Horse liver aldehyde dehydroge ...
Equus caballus
J. Biol. Chem.
251
236-240
1976
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2
17
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4
1
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3
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12
1
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1
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1
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2
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17
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4
1
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1
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1
3
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12
1
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